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Genetic Characterization of Antimicrobial Resistance in Canadian Isolates of Salmonella Serovar Typhimurium DT104

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Ng, Lai-King, Mulvey, Michael R., Martin, Irene, Peters, Geoffrey A., Johnson, Wendy·1999·Antimicrobial Agents and Chemotherapy

Abstract

PCR was used to identify antibiotic resistance determinants in 31 Canadian Salmonella serovar Typhimurium DT104 isolates. Genes encoding resistance to ampicillin ( pse1 or blaP1 ), chloramphenicol ( pasppflo -like), streptomycin-spectinomycin ( aadA2 ), sulfonamide ( sulI ), and tetracycline [ tet (G)] were mapped to a 13-kb region of DNA of one isolate. Two copies of sulI were identified and mapped to the 3′ end of either pse1 or aadA2 integrons. The two integrons were separated by the pasppflo -like gene and the tet (G) gene. The kanamycin resistance determinant ( aphA-1 ) was present on a 2.0-MDa plasmid (five isolates) or on the chromosome (three isolates).

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Metadata

Authors
Ng, L.-K., Mulvey, M. R., Martin, I., Peters, G. A., & Johnson, W.
Venue
Antimicrobial Agents and Chemotherapy
Published
1999-12
Volume
43
Issue
12
Pages
3018-3021
ISSN
  • 0066-4804 (ISSN-L)
  • 1098-6596
OpenAlex
W2170106191
Cited by
185
References
0
FWCI
13.94
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Genetic Characterization of Antimicrobial Resistance in Canadian Isolates of Salmonella Serovar Typhimurium DT104. https://app.cochat.ai/papers/10.1128/aac.43.12.3018
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Ng, L.-K., Mulvey, M. R., Martin, I., Peters, G. A., & Johnson, W. (1999). Genetic Characterization of Antimicrobial Resistance in Canadian Isolates of Salmonella Serovar Typhimurium DT104. Antimicrobial Agents and Chemotherapy. https://doi.org/10.1128/aac.43.12.3018

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