Genetic Analysis of Multiple Loci Suggests that Mutations in the Pneumocystis carinii f. sp. hominis Dihydropteroate Synthase Gene Arose Independently in Multiple Strains
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To determine if mutations in the dihydropteroate synthase (DHPS) gene of Pneumocystis carinii f. sp. hominis arose in a single strain that was subsequently widely disseminated, we examined four genomic regions of 22 P. carinii clinical isolates selected based on the absence or presence of mutations in the DHPS gene. By single-strand conformation polymorphism and DNA sequencing, we found varying genotypes for each of the four regions in isolates with DHPS mutations, suggesting that these mutations occurred independently in multiple strains of P. carinii. This suggests that exposure to sulfa will select for these mutations in diverse strains.
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Genetic analysis suggests that the mutations arose independently in multiple strains of Pneumocystis, which supports the theory that exposure to sulfa medications selects for DHPS gene mutations.
“Genetic analysis suggests that the mutations arose independently in multiple strains of Pneumocystis, which supports the theory that exposure to sulfa medications selects for DHPS gene mutations (26).”
From Dihydropteroate Synthase Gene Mutations in<i>Pneumocystis</i>and Sulfa Resistance (2004) · cites this paper as [26]
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References 17 · Cited by 34
- Authors
- Ma, L., & Kovacs, J. A.
- Affiliations
- National Institutes of Health (US)
- Venue
- Antimicrobial Agents and Chemotherapy
- Publisher
- American Society for Microbiology
- Published
- 2001-11
- Volume
- 45
- Issue
- 11
- Pages
- 3213-3215
- ISSN
- 0066-4804 (ISSN-L)
- 1098-6596
- PMC
- PMC90808
- OpenAlex
- W2167021182
- Cited by
- 34
- References
- 17
- Citations by year
- 2026: 1 · 2023: 1 · 2021: 2 · 2020: 1 · 2019: 3 · 2018: 1 · 2017: 2 · 2016: 2 · 2015: 1 · 2012: 1
- FWCI
- 3.05
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Antimicrobial Agents and Chemotherapy (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Epidemiology › Pneumocystis jirovecii pneumonia detection and treatment
- Health Sciences › Medicine › Epidemiology › Pneumonia and Respiratory Infections
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
- Keywords
- dihydropteroate synthase, dhps mutations, genetic diversity, clinical isolates, sulfonamide resistance, falciparum malaria
- MeSH terms
- Alleles, Dihydropteroate Synthase, DNA, Fungal, Genotype, Mutation, Pneumocystis, RNA, Fungal, Polymorphism, Single-Stranded Conformational, Reverse Transcriptase Polymerase Chain Reaction
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