In Vitro Activity of Caspofungin against Candida albicans Biofilms
Open accessAbstract
Most manifestations of candidiasis are associated with biofilm formation on biological or inanimate surfaces. Candida albicans biofilms are recalcitrant to treatment with conventional antifungal therapies. Here we report on the activity of caspofungin, a new semisynthetic echinocandin, against C. albicans biofilms. Caspofungin displayed potent in vitro activity against sessile C. albicans cells within biofilms, with MICs at which 50% of the sessile cells were inhibited well within the drug's therapeutic range. Scanning electron microscopy and confocal scanning laser microscopy were used to visualize the effects of caspofungin on preformed C. albicans biofilms, and the results indicated that caspofungin affected the cellular morphology and the metabolic status of cells within the biofilms. The coating of biomaterials with caspofungin had an inhibitory effect on subsequent biofilm development by C. albicans . Together these findings indicate that caspofungin displays potent activity against C. albicans biofilms in vitro and merits further investigation for the treatment of biofilm-associated infections.
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Early work in the late 1990s and early 2000s demonstrated potent antifungal activity of physiological concentrations of echinocandins against C. albicans biofilms, with subsequent studies extended to other Candida spp.
“Early work in these academic laboratories in the late 1990s and early 2000s demonstrated the potent antifungal activity of physiological concentrations of echinocandins against C. albicans biofilms, with subsequent studies extended to other Candida spp [34,37,38].”
From Antifungal therapy of Candida biofilms: Past, present and future (2023) · cites this paper as [37]
Cells in biofilms have been found to be more resistant to drugs compared with cells grown in suspension culture.
“For example, a number of these biofilm formers (Table 3) have been tested for drug susceptibility in vitro, and cells in biofilms have been found to be more resistant to drugs compared with cells grown in suspension culture.”
From <i>Candida albicans</i> Biofilms and Human Disease (2015) · cites this paper as [9]
Members from Acremonium, Aspergillus, Cladosporium, Fusarium, and Pneumocystis, in addition to Candida, are among the biofilm formers tested for drug susceptibility in vitro.
“These include (in addition to Candida) members from Acremonium, Aspergillus, Cladosporium, Fusarium, and Pneumocystis (9, 20, 35, 42, 79, 160, 176).”
From <i>Candida albicans</i> Biofilms and Human Disease (2015) · cites this paper as [9]
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References 43 · Cited by 288
Research works printed but not linked: 1
- [22]Hawser, S. 1996. Comparisons of the susceptibilities of planktonic and adherent Candida albicans to antifungal agents: a modified XTT tetrazolium assay using synchronised C. albicans cells. J. Med. Vet. Mycol. 34:149-152. [PubMed] [Google Scholar]
- Authors
- Bachmann, S. P., VandeWalle, K., Ramage, G., Patterson, T. F., Wickes, B. L., Graybill, J. R., & López-Ribot, J. L.
- Affiliations
- Department of Medicine, Division of Infectious Diseases
- The University of Texas at San Antonio Health Science Center (US)
- Venue
- Antimicrobial Agents and Chemotherapy
- Publisher
- American Society for Microbiology
- Published
- 2002-11
- Volume
- 46
- Issue
- 11
- Pages
- 3591-3596
- ISSN
- 0066-4804 (ISSN-L)
- 1098-6596
- PMC
- PMC128731
- OpenAlex
- W2034519764
- Cited by
- 288
- References
- 43
- Citations by year
- 2025: 4 · 2024: 3 · 2023: 7 · 2022: 4 · 2021: 13 · 2020: 13 · 2019: 6 · 2018: 9 · 2017: 12 · 2016: 14 · 2015: 17 · 2014: 14 · 2013: 18 · 2012: 11
- FWCI
- 7.73
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Antimicrobial Agents and Chemotherapy (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (submittedVersion)
- Funders
- National Institute of Dental and Craniofacial Research (5 R01 DE11381, R01DE011381)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- caspofungin, Candida albicans, biofilm, echinocandins, candidiasis, antifungal susceptibility, cell morphology, biofilm-associated infections, biomaterial coatings, confocal laser scanning microscopy
- MeSH terms
- Caspofungin, Anti-Bacterial Agents, Candida albicans, Microbial Sensitivity Tests, Microscopy, Electron, Peptides, Peptides, Cyclic, Biofilms, Echinocandins, Lipopeptides
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