Resazurin Microtiter Assay Plate: Simple and Inexpensive Method for Detection of Drug Resistance in Mycobacterium tuberculosis
Open accessAbstract
A method for detecting multidrug-resistant Mycobacterium tuberculosis by using a reduction of resazurin is described. Eighty clinical isolates were evaluated against isoniazid and rifampin; results at 7 days were compared with those of the proportion method. Specificity and sensitivity were excellent. The method is simple, inexpensive, and rapid and might be used with other antituberculosis drugs.
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Preliminary studies of DST of MDR TB isolates using MTT, Alamar blue, or resazurin showed a good correlation with the conventional proportion method.
“Preliminary studies of DST of MDR TB isolates using MTT, Alamar blue, or resazurin showed a good correlation with the conventional PM (1, 13, 19, 20, 31, 37, 49).”
From Resazurin Microtiter Assay Plate Testing of <i>Mycobacterium tuberculosis</i> Susceptibilities to Second-Line Drugs: Rapid, Simple, and Inexpensive Method (2003) · cites this paper as [37]
The REMA plate method has proven to be, in recent experience, a reliable method for the detection of MDR TB.
“The REMA plate method has proven to be, in recent experience, a reliable method for the detection of MDRTB(37).”
From Resazurin Microtiter Assay Plate Testing of <i>Mycobacterium tuberculosis</i> Susceptibilities to Second-Line Drugs: Rapid, Simple, and Inexpensive Method (2003) · cites this paper as [37]
The authors previously developed a REMA plate for detecting MDR TB and demonstrated a very good correlation between results by this method and the PM.
“Since resazurin has been recently identified as the main component of Alamar blue (34, 43), we recently developed a resazurin microtiter assay (REMA) plate (37) for detecting MDR TB and demonstrated a very good correlation between results by this method and the proportion method (PM).”
From Resazurin Microtiter Assay Plate Testing of <i>Mycobacterium tuberculosis</i> Susceptibilities to Second-Line Drugs: Rapid, Simple, and Inexpensive Method (2003) · cites this paper as [37]
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Research works printed but not linked: 1
- [30]30. Takiff, H. 2000. Molecular mechanisms of drug resistance, p. 77-114. In I. Bastian and F. Portaels (ed.), Multidrug-resistant tuberculosis. Kluwer Academic Publishers, Dordrecht, The Netherlands.
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Martin, A., Camacho, M., Portaels, F., & Palomino, J. C. (2003) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.47.11.3616-3619.2003 · Cited by 306 · see citing works
Self-citationAddressing the Metabolic Stability of Antituberculars through Machine Learning
Stratton, T. P., Perryman, A. L., Vilchèze, C., Russo, R., Li, S.-G., Patel, J. S., Singleton, E., Ekins, S., Connell, N., Jacobs, W. R., et al. (2017) · ACS Medicinal Chemistry Letters · doi:10.1021/acsmedchemlett.7b00299 · Cited by 14 · see citing works
- Authors
- Palomino, J.-C., Martin, A., Camacho, M., Guerra, H., Swings, J., & Portaels, F.
- Affiliations
- Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000
- Instituut voor Tropische Geneeskunde (BE)
- Ministerio de Salud (BO)
- Universidad Peruana Cayetano Heredia (PE)
- Ghent University (BE)
- Venue
- Antimicrobial Agents and Chemotherapy
- Publisher
- American Society for Microbiology
- Published
- 2002-08
- Volume
- 46
- Issue
- 8
- Pages
- 2720-2722
- ISSN
- 0066-4804 (ISSN-L)
- 1098-6596
- PMC
- PMC127336
- OpenAlex
- W2116128143
- Cited by
- 1,229
- References
- 0
- Citations by year
- 2026: 54 · 2025: 75 · 2024: 72 · 2023: 88 · 2022: 77 · 2021: 80 · 2020: 86 · 2019: 90 · 2018: 91 · 2017: 82 · 2016: 60 · 2015: 54 · 2014: 56 · 2013: 42 · 2012: 51
- FWCI
- 3.74
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Antimicrobial Agents and Chemotherapy (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Ghent University Academic Bibliography (Ghent University) (submittedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Health Sciences › Medicine › Surgery › Infectious Diseases and Tuberculosis
- Health Sciences › Medicine › Epidemiology › Mycobacterium research and diagnosis
- Keywords
- Mycobacterium tuberculosis, multidrug resistance, resazurin, microtiter plate assay, isoniazid resistance, rifampicin resistance, drug resistance detection, rapid diagnostic tests
- MeSH terms
- Antitubercular Agents, Colorimetry, Drug Resistance, Microbial, Humans, Indicators and Reagents, Isoniazid, Microbial Sensitivity Tests, Mycobacterium tuberculosis, Oxazines, Peru, Rifampin, Tuberculosis, Xanthenes
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