CoChat
Sign Up
Cited by 286Antibiotic Resistance in BacteriaBacterial Genetics and BiotechnologyDrug Transport and Resistance Mechanisms

The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.

Efflux-Mediated Resistance to Tigecycline (GAR-936) in Pseudomonas aeruginosa PAO1

Open access
Dean, Charles R., Visalli, Melissa A., Projan, Steven J., Sum, Phaik-Eng, Bradford, Patricia A.·2003·Antimicrobial Agents and Chemotherapy
DOI 10.1128/aac.47.3.972-978.2003PMC PMC149306
Open this paper in CoChat — free account

Abstract

Pseudomonas aeruginosa strains are less susceptible to tigecycline (previously GAR-936; MIC, 8 μg/ml) than many other bacteria (P. J. Petersen, N. V. Jacobus, W. J. Weiss, P. E. Sum, and R. T. Testa, Antimicrob. Agents Chemother. 43:738-744, 1999). To elucidate the mechanism of resistance to tigecycline, P. aeruginosa PAO1 strains defective in the MexAB-OprM and/or MexXY (OprM) efflux pumps were tested for susceptibility to tigecycline. Increased susceptibility to tigecycline (MIC, 0.5 to 1 μg/ml) was specifically associated with loss of MexXY. Transcription of mexX and mexY was also responsive to exposure of cells to tigecycline. To test for the emergence of compensatory efflux pumps in the absence of MexXY-OprM, mutants lacking MexXY-OprM were plated on medium containing tigecycline at 4 or 6 μg/ml. Resistant mutants were readily recovered, and these also had decreased susceptibility to several other antibiotics, suggesting efflux pump recruitment. One representative carbenicillin-resistant strain overexpressed OprM, the outer membrane channel component of the MexAB-OprM efflux pump. The mexAB-oprM repressor gene, mexR , from this strain contained a 15-bp in-frame deletion. Two representative chloramphenicol-resistant strains showed expression of an outer membrane protein slightly larger than OprM. The mexCD-OprJ repressor gene, nfxB , from these mutants contained a 327-bp in-frame deletion and an IS element insertion, respectively. Together, these data indicated drug efflux mediated by MexCD-OprJ. The MICs of the narrower-spectrum semisynthetic tetracyclines doxycycline and minocycline increased more substantially than did those of tigecycline and other glycylcyclines against the MexAB-OprM- and MexCD-OprJ-overexpressing mutant strains. This suggests that glycylcyclines, although they are subject to efflux from P. aeruginosa , are generally inferior substrates for P. aeruginosa efflux pumps than are narrower-spectrum tetracyclines.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1128/aac.47.3.972-978.2003

Cites

43 of 43 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Cited by

Sorted by citations

See all 286

Related

See all

Metadata

Authors
Dean, C. R., Visalli, M. A., Projan, S. J., Sum, P.-E., & Bradford, P. A.
Affiliations
Wyeth (US)
Corresponding author
Dean, Charles R.
ORCID
Venue
Antimicrobial Agents and Chemotherapy
Publisher
American Society for Microbiology
Published
2003-03
Volume
47
Issue
3
Pages
972-978
ISSN
  • 0066-4804 (ISSN-L)
  • 1098-6596
DOI
10.1128/aac.47.3.972-978.2003
PMC
PMC149306
OpenAlex
W2139818499
Cited by
286
References
43
Citations by year
2026: 8 · 2025: 7 · 2024: 4 · 2023: 6 · 2022: 13 · 2021: 14 · 2020: 8 · 2019: 9 · 2018: 10 · 2017: 6 · 2016: 14 · 2015: 9 · 2014: 8 · 2013: 10 · 2012: 12
FWCI
9.42
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Antimicrobial Agents and Chemotherapy (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
Keywords
tigecycline resistance, Pseudomonas aeruginosa PAO1, efflux pumps, MexXY-OprM, MexCD-OprJ, MexAB-OprM, OprM, mexR, glycylcyclines, tetracycline, doxycycline, minocycline, carbenicillin resistance
MeSH terms
Tigecycline, Anti-Bacterial Agents, Bacterial Outer Membrane Proteins, Bacterial Proteins, Culture Media, DNA, Bacterial, Electrophoresis, Polyacrylamide Gel, Microbial Sensitivity Tests, Minocycline, Pseudomonas aeruginosa, Tetracycline Resistance, Tetracyclines, Transcription, Genetic, Blotting, Western, Membrane Transport Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review