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Rapid Diffusion of Fluorescent Tracers into Staphylococcus epidermidis Biofilms Visualized by Time Lapse Microscopy
Open accessAbstract
The transient diffusion of fluorescent tracers into biofilm cell clusters of Staphylococcus epidermidis was visualized by time lapse confocal scanning laser microscopy. Rhodamine B diffused into the center of cell clusters that were 200 to 600 μm in diameter within a few minutes. The apparent effective diffusion coefficient calculated from these data averaged 3.7 × 10 −7 cm 2 s −1 or 11% of the value in pure water. Fluorescein diffused into biofilm more rapidly, with a diffusion coefficient that averaged 1.6 × 10 −6 cm 2 s −1 , or 32% of the value in water. This study provides direct, visual confirmation that solutes the size of many antibiotics and biocides can diffuse rapidly into biofilms.
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Cited by
Physiological heterogeneity in biofilms
Stewart, P. S., & Franklin, M. J. (2008) · Nature Reviews Microbiology · doi:10.1038/nrmicro1838 · Cited by 2,327
Self-citationPenetration of antibiotics through Staphylococcus aureus and Staphylococcus epidermidis biofilms
Singh, R., Ray, P., Das, A., & Sharma, M. (2010) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkq257 · Cited by 422
Majumder, M., Chopra, N., & Hinds, B. J. (2011) · ACS Nano · doi:10.1021/nn200222g · Cited by 351
Photodynamic therapy in the control of oral biofilms
Soukos, N. S., & Goodson, J. M. (2011) · Periodontology 2000 · doi:10.1111/j.1600-0757.2010.00346.x · Cited by 342
Inorganic nanoparticles engineered to attack bacteria
Miller, K. P., Wang, L., Benicewicz, B. C., & Decho, A. W. (2015) · Chemical Society Reviews · doi:10.1039/c5cs00041f · Cited by 290
Metadata
- Authors
- Rani, S. A., Pitts, B., & Stewart, P. S.
- Venue
- Antimicrobial Agents and Chemotherapy
- Publisher
- American Society for Microbiology
- Published
- 2005-02
- Volume
- 49
- Issue
- 2
- Pages
- 728-732
- ISSN
- 0066-4804 (ISSN-L)
- 1098-6596
- OpenAlex
- W2108905378
- Cited by
- 185
- References
- 0
- FWCI
- 3.00
- Type
- journal article
- Language
- en
- Open access
- green
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- Rani, S. A., Pitts, B., & Stewart, P. S. (2005). Rapid Diffusion of Fluorescent Tracers into Staphylococcus epidermidis Biofilms Visualized by Time Lapse Microscopy. Antimicrobial Agents and Chemotherapy. https://doi.org/10.1128/aac.49.2.728-732.2005
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