The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Purification and Characterization of OmcZ, an Outer-Surface, Octaheme c -Type Cytochrome Essential for Optimal Current Production by Geobacter sulfurreducens
Open accessAbstract
Previous studies have demonstrated that Geobacter sulfurreducens requires the c -type cytochrome OmcZ, which is present in large (OmcZ L ; 50-kDa) and small (OmcZ S ; 30-kDa) forms, for optimal current production in microbial fuel cells. This protein was further characterized to aid in understanding its role in current production. Subcellular-localization studies suggested that OmcZ S was the predominant extracellular form of OmcZ. N- and C-terminal amino acid sequence analysis of purified OmcZ S and molecular weight measurements indicated that OmcZ S is a cleaved product of OmcZ L retaining all 8 hemes, including 1 heme with the unusual c -type heme-binding motif CX 14 CH. The purified OmcZ S was remarkably thermally stable (thermal-denaturing temperature, 94.2°C). Redox titration analysis revealed that the midpoint reduction potential of OmcZ S is approximately −220 mV (versus the standard hydrogen electrode [SHE]) with nonequivalent heme groups that cover a large reduction potential range (−420 to −60 mV). OmcZ S transferred electrons in vitro to a diversity of potential extracellular electron acceptors, such as Fe(III) citrate, U(VI), Cr(VI), Au(III), Mn(IV) oxide, and the humic substance analogue anthraquinone-2,6-disulfonate, but not Fe(III) oxide. The biochemical properties and extracellular localization of OmcZ suggest that it is well suited for promoting electron transfer in current-producing biofilms of G. sulfurreducens .
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Measurement of protein using bicinchoninic acid
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Humic substances as electron acceptors for microbial respiration
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Self-citationElectrode-Reducing Microorganisms That Harvest Energy from Marine Sediments
Bond, D. R., Holmes, D. E., Tender, L. M., & Lovley, D. R. (2002) · Science · doi:10.1126/science.1066771 · Cited by 1,437
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Self-citationNevin, K. P., Kim, B.-C., Glaven, R. H., Johnson, J. P., Woodard, T. L., Methé, B. A., DiDonato, R. J., Covalla, S. F., Franks, A. E., Liu, A., et al. (2009) · PLoS ONE · doi:10.1371/journal.pone.0005628 · Cited by 441
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Kracke, F., Vassilev, I., & Krömer, J. O. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.00575 · Cited by 748
Alignment of the c -Type Cytochrome OmcS along Pili of Geobacter sulfurreducens
Leang, C., Qian, X., Mester, T., & Lovley, D. R. (2010) · Applied and Environmental Microbiology · doi:10.1128/aem.00023-10 · Cited by 342
Self-citationMicrobial Nanowires: A New Paradigm for Biological Electron Transfer and Bioelectronics
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Metadata
- Authors
- Inoue, K., Qian, X., Morgado, L., Kim, B.-C., Mester, T., Izallalen, M., Salgueiro, C. A., & Lovley, D. R.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2010-06-15
- Volume
- 76
- Issue
- 12
- Pages
- 3999-4007
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2031188248
- Cited by
- 265
- References
- 6
- FWCI
- 18.57
- Type
- journal article
- Language
- en
- Open access
- green
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- The paper
- Inoue, K., Qian, X., Morgado, L., Kim, B.-C., Mester, T., Izallalen, M., Salgueiro, C. A., & Lovley, D. R. (2010). Purification and Characterization of OmcZ, an Outer-Surface, Octaheme c -Type Cytochrome Essential for Optimal Current Production by Geobacter sulfurreducens. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.00027-10
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