The DOI matches its Crossref record.
Cloning of a Novel Pyrethroid-Hydrolyzing Carboxylesterase Gene from Sphingobium sp. Strain JZ-1 and Characterization of the Gene Product
Open accessAbstract
A novel esterase gene, pytH , encoding a pyrethroid-hydrolyzing carboxylesterase was cloned from Sphingobium sp. strain JZ-1. The gene contained an open reading frame of 840 bp. Sequence identity searches revealed that the deduced enzyme shared the highest similarity with many α/β-hydrolase fold proteins (20 to 24% identities). PytH was expressed in Escherichia coli BL21 and purified using Ni-nitrilotriacetic acid affinity chromatography. It was a monomeric structure with a molecular mass of approximately 31 kDa and a pI of 4.85. PytH was able to transform p -nitrophenyl esters of short-chain fatty acids and a wide range of pyrethroid pesticides, and isomer selectivity was not observed. No cofactors were required for enzyme activity.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Insight Into Microbial Applications for the Biodegradation of Pyrethroid Insecticides
Bhatt, P., Huang, Y., Zhan, H., & Chen, S. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.01778 · Cited by 202
Chen, S., Dong, Y. H., Chang, C., Deng, Y., Zhang, X. F., Zhong, G., Song, H., Hu, M., & Zhang, L.-H. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2013.01.002 · Cited by 138
Russell, R. J., Scott, C., Jackson, C. J., Pandey, R., Pandey, G., Taylor, M. C., Coppin, C. W., Liu, J., & Oakeshott, J. G. (2011) · Evolutionary Applications · doi:10.1111/j.1752-4571.2010.00175.x · Cited by 130
Metadata
- Authors
- Wang, B.-Z., Guo, P., Hang, B.-J., Li, L., He, J., & Li, S.-P.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2009-09
- Volume
- 75
- Issue
- 17
- Pages
- 5496-5500
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2097753075
- Cited by
- 162
- References
- 0
- FWCI
- 6.53
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Cloning of a Novel Pyrethroid-Hydrolyzing Carboxylesterase Gene from Sphingobium sp. Strain JZ-1 and Characterization of the Gene Product. https://app.cochat.ai/papers/10.1128/aem.01298-09
- The paper
- Wang, B.-Z., Guo, P., Hang, B.-J., Li, L., He, J., & Li, S.-P. (2009). Cloning of a Novel Pyrethroid-Hydrolyzing Carboxylesterase Gene from Sphingobium sp. Strain JZ-1 and Characterization of the Gene Product. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.01298-09
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
