The DOI matches its Crossref record.
Quantification of the Detrimental Effect of a Single Primer-Template Mismatch by Real-Time PCR Using the 16S rRNA Gene as an Example
Open accessAbstract
We investigated the effects of internal primer-template mismatches on the efficiency of PCR amplification using the 16S rRNA gene as the model template DNA. We observed that the presence of a single mismatch in the second half of the primer extension sequence can result in an underestimation of up to 1,000-fold of the gene copy number, depending on the primer and position of the mismatch.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Apprill, A., McNally, S., Parsons, R., & Weber, L. (2015) · Aquatic Microbial Ecology · doi:10.3354/ame01753 · Cited by 2,707
Stadhouders, R., Pas, S. D., Anber, J., Voermans, J., Mes, T. H. M., & Schutten, M. (2010) · The Journal of Molecular Diagnostics · doi:10.2353/jmoldx.2010.090035 · Cited by 399
Resolving rates of mutation in the brain using single-neuron genomics
Evrony, G. D., Lee, E., Park, P. J., & Walsh, C. A. (2016) · eLife · doi:10.7554/elife.12966 · Cited by 163
Regulation of bacterial and fungal MCPA degradation at the soil–litter interface
Poll, C., Pagel, H., Devers-Lamrani, M., Martin-Laurent, F., Ingwersen, J., Streck, T., & Kandeler, E. (2010) · Soil Biology and Biochemistry · doi:10.1016/j.soilbio.2010.07.013 · Cited by 48
Self-citation
Metadata
- Authors
- Bru, D., Martin-Laurent, F., & Philippot, L.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2008-03
- Volume
- 74
- Issue
- 5
- Pages
- 1660-1663
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2151308724
- Cited by
- 284
- References
- 0
- FWCI
- 4.84
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
