Formation of In Vitro Mixed-Species Biofilms by Lactobacillus pentosus and Yeasts Isolated from Spanish-Style Green Table Olive Fermentations
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The present work details the in vitro interactions between Lactobacillus pentosus and yeast strains isolated from table olive processing to form mixed biofilms. Among the different pairs assayed, the strongest biofilms were obtained from L. pentosus and Candida boidinii strain cocultures. However, biofilm formation was inhibited in the presence of d -(+)-mannose. In addition, biofilm formation by C. boidinii monoculture was stimulated in the absence of cell-cell contact with L. pentosus . Scanning electron microscopy revealed that a sort of “sticky” material formed by the yeasts contributed to substrate adherence. Hence, the data obtained in this work suggest that yeast-lactobacilli biofilms may be favored by the presence of a specific mate of yeast and L. pentosus , and that more than one mechanism might be implicated in the biofilm formation. This knowledge will help in the design of appropriate mixed starter cultures of L. pentosus -yeast species pairs that are able to improve the quality and safety of Spanish-style green table olive processing.
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Mannose-specific adhesins reduce pathogen colonization due to co-aggregation with mannose-containing surface structures of the pathogen.
“Furthermore, mannose-specific adhesins reduce pathogen colonization due to co-aggregation with mannose-containing surface structures of the pathogen [52–54].”
From Bistable auto-aggregation phenotype in Lactiplantibacillus plantarum emerges after cultivation in in vitro colonic microbiota (2021) · cites this paper as [54]
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References 33 · Cited by 38
- Authors
- León-Romero, Á., Domínguez-Manzano, J., Garrido-Fernández, A., Arroyo-López, F. N., & Jiménez-Díaz, R.
- Affiliations
- Consejo Superior de Investigaciones Científicas (ES)
- Food Biotechnology Department, Instituto de la Grasa, Agencia Estatal Consejo Superior de Investigaciones Científicas (CSIC), University Campus Pablo de Olavide, Seville, Spain
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2016-01-15
- Volume
- 82
- Issue
- 2
- Pages
- 689-695
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC4711112
- OpenAlex
- W2228231556
- Cited by
- 38
- References
- 33
- Citations by year
- 2025: 1 · 2024: 3 · 2023: 2 · 2022: 4 · 2021: 4 · 2020: 6 · 2019: 10 · 2018: 3 · 2017: 3 · 2016: 1 · 2013: 1
- FWCI
- 3.12
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- Funders
- MINECO | Consejo Superior de Investigaciones Científicas (AGL2009-08016/ALI, AGL2010-15529/ALI, AGL2013-48300-R/ALI)
- Consejería de Economía, Innovación, Ciencia y Empleo, Junta de Andalucía (P11-AGR-7051)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Agricultural and Biological Sciences › Food Science › Fermentation and Sensory Analysis
- Keywords
- Lactobacillus pentosus, polymicrobial biofilms, Candida boidinii, mannose inhibition, cell-cell contact, starter cultures
- MeSH terms
- Fermentation, Lactobacillus, Spain, Yeasts, Biofilms, Olea
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