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Cited by 161Microbial Natural Products and BiosynthesisFluid Dynamics and MixingMicrobial Metabolic Engineering and Bioproduction

The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.

Effect of Growth Rate on the Synthesis of Penicillin by Penicillium chrysogenum in Batch and Chemostat Cultures

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Pirt, S. J., Righelato, R. C.·1967·Applied Microbiology
DOI 10.1128/aem.15.6.1284-1290.1967PMC PMC547184
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Abstract

The kinetics of penicillin production by Penicillium chrysogenum Wis 54-1255 in a glucose-limited chemostat and in batch cultures are reported. The specific production rate of penicillin, q pen (units per milligram of dry weight per hour) was independent of specific growth rate over the range 0.014 to 0.086 hr -1 . Growth was stopped by restricting the glucose supply to the “maintenance ration,” that is, the glucose requirement of the organism at zero growth rate with all other nutrients in excess. Under such conditions, the organism dry weight remained constant, but the q pen fell approximately linearly to zero at a rate inversely related to the previous growth rate. Glucose supplied in excess of the maintenance ration inhibited the decay of q pen . At a critical growth rate between 0.009 and 0.014 hr -1 , the decay was completely inhibited. Quantitative expressions for the q pen of growing and nongrowing cultures were derived and used to predict the steady-state concentrations of penicillin accumulating in one- and two-stage continuous processes. A rational explanation of the kinetics of penicillin accumulation in batch cultures is given, relating the rate of penicillin synthesis to growth rate. It is concluded that an important role of corn steep liquor (CSL), a heterogeneous carbon and nitrogen source commonly used in penicillin production media, is the provision of substrates which allow a high concentration of mold to be reached before the growth rate falls below the critical value. CSL had no significant effect on q pen .

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Crossref · 10.1128/am.15.6.1284-1290.1967

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Metadata

Authors
Pirt, S. J., & Righelato, R. C.
Affiliations
University of London (GB)
Venue
Applied Microbiology
Publisher
American Society for Microbiology
Published
1967-11
Volume
15
Issue
6
Pages
1284-1290
ISSN
0003-6919 (ISSN-L)
DOI
10.1128/aem.15.6.1284-1290.1967
PMC
PMC547184
OpenAlex
W1523255283
Semantic Scholar
d005e9d37baf70e78b634df2656e073d1bc7d3c7
Cited by
161
References
8
Citations by year
2026: 2 · 2025: 1 · 2024: 3 · 2022: 2 · 2021: 2 · 2020: 2 · 2019: 3 · 2017: 4 · 2016: 3 · 2014: 2 · 2013: 1 · 2012: 1
FWCI
3.18
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Applied Microbiology (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (submittedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Microbial Natural Products and Biosynthesis
  • Physical Sciences › Chemical Engineering › Fluid Flow and Transfer Processes › Fluid Dynamics and Mixing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords
penicillin production, Penicillium chrysogenum, specific growth rate, glucose limitation, maintenance ration, continuous fermentation, corn steep liquor, batch culture, chemostat

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