The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressureized atmosphere
Open accessAbstract
The sensitivity of the requirement of Methanobacterium ruminantium strain M1 to a new coenzyme, 2-mercaptoethanesulfonic acid (HS-CoM) was examined by use of new techniques that were developed for rapid and efficient handling of large numbers of cultures of methanogenic bacteria. The system uses sealed tubes that contain a gas mixture of 80% hydrogen and 20% carbon dioxide under a pressure of 2 to 3 atm. This modification of the Hungate technique reduces variability among replicate cultures and simplifies the dispensing, sterilization, and storage of liquid media as well as the transfer and maintenance of methanogenic bacteria. Results indicate a limit of sensitivity of the assay at 5 nM HS-CoM, with half-maximal growth at 25 nM HS-CoM. Coenzyme activity could be replaced by 2,2'-dithiodiethanesulfonic acid at a half-molar equivalent of the HS-CoM concentration, or by 2-(methylthio)ethanesulfonic acid on an equimolar basis. These data reveal a very sensitive and precise requirement for HS-CoM in the nutrition of this fastidious anaerobe.
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Cites
Holdeman, L. V., Moore, W. E., & Cato, E. P. (1977) · Cited by 2,988
Chapter IV A Roll Tube Method for Cultivation of Strict Anaerobes
Hungate, R. E. (1969) · Methods in Microbiology · doi:10.1016/s0580-9517(08)70503-8 · Cited by 1,343
THE ANAEROBIC MESOPHILIC CELLULOLYTIC BACTERIA
Hungate, R. E. (1950) · Bacteriological Reviews · doi:10.1128/br.14.1.1-49.1950 · Cited by 981
Commentary on the Hungate technique for culture of anaerobic bacteria
Bryant, M. P. (1972) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/25.12.1324 · Cited by 889
A Serum Bottle Modification of the Hungate Technique for Cultivating Obligate Anaerobes
Miller, T. L., & Wolin, M. J. (1974) · Applied Microbiology · doi:10.1128/aem.27.5.985-987.1974 · Cited by 875
Bryant, M. P., & Robinson, I. M. (1961) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(61)89906-2 · Cited by 363
Structure and Methylation of Coenzyme M (HSCH2CH2SO3)
Taylor, C. D., & Wolfe, R. S. (1974) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)42403-4 · Cited by 252
Self-citationUse of anaerobic glove boxes for the cultivation of strictly anaerobic bacteria
Aranki, A., & Freter, R. (1972) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/25.12.1329 · Cited by 200
Use of syringe methods for anaerobiosis
Macy, J. M., Snellen, J. E., & Hungate, R. E. (1972) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/25.12.1318 · Cited by 192
Coenzyme M, Essential for Growth of a Rumen Strain of Methanobacterium ruminantium
Taylor, C. D., McBride, B. C., WOLFE, R. S., & Bryant, M. P. (1974) · Journal of Bacteriology · doi:10.1128/jb.120.2.974-975.1974 · Cited by 86
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Cited by
Theriot, C. M., Koenigsknecht, M. J., Carlson, P. E., Hatton, G. E., Nelson, A. M., Li, B., Huffnagle, G. B., Z. Li, J., & Young, V. B. (2014) · Nature Communications · doi:10.1038/ncomms4114 · Cited by 974
Caccavo, F., Lonergan, D. J., Lovley, D. R., Davis, M., Stolz, J. F., & McInerney, M. J. (1994) · Applied and Environmental Microbiology · doi:10.1128/aem.60.10.3752-3759.1994 · Cited by 896
Jones, W. J., Leigh, J. A., Mayer, F., Woese, C. R., & Wolfe, R. S. (1983) · Archives of Microbiology · doi:10.1007/bf00425213 · Cited by 573
Self-citationLeahy, S. C., Kelly, W. J., Altermann, E., Ronimus, R. S., Yeoman, C. J., Pacheco, D. M., Li, D., Kong, Z., McTavish, S., Sang, C., et al. (2010) · PLoS ONE · doi:10.1371/journal.pone.0008926 · Cited by 320
Bacterial Methanogenesis and Growth from CO 2 with Elemental Iron as the Sole Source of Electrons
Daniels, L., Belay, N., Rajagopal, B. S., & Weimer, P. J. (1987) · Science · doi:10.1126/science.237.4814.509 · Cited by 287
Metadata
- Authors
- Balch, W. E., & Wolfe, R. S.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1976-12
- Volume
- 32
- Issue
- 6
- Pages
- 781-791
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1560139137
- Cited by
- 916
- References
- 11
- FWCI
- 87.98
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressureized atmosphere. https://app.cochat.ai/papers/10.1128/aem.32.6.781-791.1976
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- Balch, W. E., & Wolfe, R. S. (1976). New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressureized atmosphere. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.32.6.781-791.1976
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