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Fluorescein Diacetate Hydrolysis as a Measure of Total Microbial Activity in Soil and Litter
Open accessAbstract
Spectrophotometric determination of the hydrolysis of fluorescein diacetate (FDA) was shown to be a simple, sensitive, and rapid method for determining microbial activity in soil and litter. FDA hydrolysis was studied in soil and straw incubated for up to 3 h. Hydrolysis was found to increase linearly with soil addition. FDA hydrolysis by pure cultures of Fusarium culmorum increased linearly with mycelium addition both in shake cultures and after inoculation into sterile soil. FDA hydrolysis by Pseudomonas denitrificans increased linearly with biomass addition. The FDA hydrolytic activities in soil samples from different layers of an agricultural soil were correlated with respiration. Acetone was found to be suitable for terminating the reaction.
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Citations
Cited by
Agronomic values of greenwaste biochar as a soil amendment
Chan, K. Y., Van Zwieten, L., Meszaros, I., Downie, A., & Joseph, S. (2007) · Soil Research · doi:10.1071/sr07109 · Cited by 1,902
B IOCONTROL W ITHIN THE C ONTEXT OF S OIL M ICROBIAL C OMMUNITIES: A Substrate-Dependent Phenomenon
Hoitink, H., & Boehm, M. (1999) · Annual Review of Phytopathology · doi:10.1146/annurev.phyto.37.1.427 · Cited by 843
Colonization, biofilm formation and biodegradation of polyethylene by a strain of Rhodococcus ruber
(Orr), I. G., Hadar, Y., & Sivan, A. (2004) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-004-1584-8 · Cited by 539
Identifying the characteristics of organic soil amendments that suppress soilborne plant diseases
Bonanomi, G., Antignani, V., Capodilupo, M., & Scala, F. (2010) · Soil Biology and Biochemistry · doi:10.1016/j.soilbio.2009.10.012 · Cited by 504
Debosz, K., Petersen, S. O., Kure, L. K., & Ambus, P. (2002) · Applied Soil Ecology · doi:10.1016/s0929-1393(01)00191-3 · Cited by 301
Metadata
- Authors
- Schn�rer, J., & Rosswall, T.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1982-06
- Volume
- 43
- Issue
- 6
- Pages
- 1256-1261
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1579784956
- Cited by
- 1,331
- References
- 0
- FWCI
- 12.16
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Fluorescein Diacetate Hydrolysis as a Measure of Total Microbial Activity in Soil and Litter. https://app.cochat.ai/papers/10.1128/aem.43.6.1256-1261.1982
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- Schn�rer, J., & Rosswall, T. (1982). Fluorescein Diacetate Hydrolysis as a Measure of Total Microbial Activity in Soil and Litter. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.43.6.1256-1261.1982
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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