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Microelectrode Studies of Interstitial Water Chemistry and Photosynthetic Activity in a Hot Spring Microbial Mat
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Microelectrodes were used to measure oxygen, pH, and oxygenic photosynthetic activity in a hot spring microbial mat (Octopus Spring, Yellowstone National Park), where the cyanobacterium Synechococcus lividus and the filamentous bacterium Chloroflexus aurantiacus are the only known phototrophs. The data showed very high biological activities in the topmost layers of the microbial mat, resulting in extreme values for oxygen and pH. At a 1-mm depth at a 55°C site, oxygen and pH reached 900 μM and 9.4, respectively, just after solar noon, whereas anoxic conditions with a pH of 7.2 were measured before sunrise. Although diurnal changes between these extremes occurred over hours during a diurnal cycle, microbial activity was great enough to give the same response in 1 to 2 min after artificial shading. Oxygenic photosynthesis was confined to a 0.5- to 1.1-mm layer at sites with temperatures at or above about 50°C, with maximum activities in the 55 to 60°C region. The data suggest that S. lividus is the dominant primary producer of the mat.
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“The metabolic activity of SRBs would have increased as DO decreased, especially at night (75).”
From Carbon Sequestration in Biogenic Magnesite and Other Magnesium Carbonate Minerals (2019) · cites this paper as [75]
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Pierpont, C. L., Baroch, J. J., Church, M. J., & Miller, S. R. (2024) · The ISME Journal · doi:10.1093/ismejo/wrae184 · Cited by 10
Abundant and active community members respond to diel cycles in hot spring phototrophic mats
Shelton, A. N., Yu, F. B., Grossman, A. R., & Bhaya, D. (2025) · The ISME Journal · doi:10.1093/ismejo/wraf001 · Cited by 8
Mathrani, I. M., Nielsen, P., Sonne-Hansen, J., Kristjánsson, J. K., & Ahring, B. K. (1993) · Applied and Environmental Microbiology · doi:10.1128/aem.59.6.1963-1965.1993 · Cited by 7
Organic nitrogen metabolism of phototrophic bacteria
Klemme, J.-H. (1989) · Antonie van Leeuwenhoek · doi:10.1007/bf00393850 · Cited by 7
Metadata
- Authors
- Revsbech, N. P., & Ward, D. M.
- Affiliations
- Montana State University (US)
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1984-08
- Volume
- 48
- Issue
- 2
- Pages
- 270-275
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC241501
- OpenAlex
- W1536293420
- Cited by
- 123
- References
- 29
- Citations by year
- 2025: 3 · 2024: 3 · 2022: 2 · 2021: 2 · 2020: 3 · 2019: 1 · 2018: 1 · 2017: 3 · 2016: 7 · 2015: 3 · 2014: 2 · 2013: 3 · 2012: 2
- FWCI
- 6.03
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Physical Sciences › Environmental Science › Environmental Engineering › Microbial Fuel Cells and Bioremediation
- Physical Sciences › Earth and Planetary Sciences › Geochemistry and Petrology › Geochemistry and Elemental Analysis
- Keywords
- hot spring microbial mats, pore water chemistry, oxygenic photosynthesis, Synechococcus lividus, Chloroflexus aurantiacus, anoxia, primary producers, Yellowstone National Park
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