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Pilot plant production of rhamnolipid biosurfactant by Pseudomonas aeruginosa
Open accessAbstract
Rhamnolipid biosurfactants were continuously produced with Pseudomonas aeruginosa on the pilot plant scale. Production and downstream processing elaborated on the laboratory scale were adapted to the larger scale. Differences in performance resulting from the scale-up are discussed. A biosurfactant concentration of approximately 2.25 g liter-1 was achieved. The biosurfactant yield on glucose was 77 mg g-1 h-1, and the productivity was 147 mg liter-1 h-1, corresponding to a daily production of 80 g of biosurfactant. The first enrichment step consisted of an adsorption chromatography which was followed by an anion-exchange chromatography. The resulting product was 90% pure, and the overall recovery of active material was above 60% with the downstream processing used.
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Citations
Cited by
Microbial production of surfactants and their commercial potential.
Desai, J. D., & Banat, I. M. (1997) · Microbiology and molecular biology reviews : MMBR · doi:10.1128/.61.1.47-64.1997 · Cited by 2,460
Towards commercial production of microbial surfactants
Mukherjee, S., Das, P., & Sen, R. (2006) · Trends in Biotechnology · doi:10.1016/j.tibtech.2006.09.005 · Cited by 766
Abalos, A., Pinazo, A., Infante, M. R., Casals, M., García, F., & Manresa, A. (2001) · Langmuir · doi:10.1021/la0011735 · Cited by 451
Methods for investigating biosurfactants and bioemulsifiers: a review
Satpute, S. K., Banpurkar, A. G., Dhakephalkar, P. K., Banat, I. M., & Chopade, B. A. (2010) · Critical Reviews in Biotechnology · doi:10.3109/07388550903427280 · Cited by 423
Metadata
- Authors
- Reiling, H. E., Thanei-Wyss, U., Guerra-Santos, L. H., Hirt, R., Käppeli, O., & Fiechter, A.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1986-05
- Volume
- 51
- Issue
- 5
- Pages
- 985-989
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1985409836
- Cited by
- 160
- References
- 0
- FWCI
- 3.86
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Pilot plant production of rhamnolipid biosurfactant by Pseudomonas aeruginosa. https://app.cochat.ai/papers/10.1128/aem.51.5.985-989.1986
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- Reiling, H. E., Thanei-Wyss, U., Guerra-Santos, L. H., Hirt, R., Käppeli, O., & Fiechter, A. (1986). Pilot plant production of rhamnolipid biosurfactant by Pseudomonas aeruginosa. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.51.5.985-989.1986
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
