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Automatic Determination of Bacterioplankton Biomass by Image Analysis
Open accessAbstract
Image analysis was applied to epifluorescense microscopy of acridine orange-stained plankton samples. A program was developed for discrimination and binary segmentation of digitized video images, taken by an ultrasensitive video camera mounted on the microscope. Cell volumes were estimated from area and perimeter of the objects in the binary image. The program was tested on fluorescent latex beads of known diameters. Biovolumes measured by image analysis were compared with directly determined carbon biomasses in batch cultures of estuarine and freshwater bacterioplankton. This calibration revealed an empirical conversion factor from biovolume to biomass of 0.35 pg of C μm −3 (± 0.03 95% confidence limit). The deviation of this value from the normally used conversion factors of 0.086 to 0.121 pg of C μm −3 is discussed. The described system was capable of measuring 250 cells within 10 min, providing estimates of cell number, mean cell volume, and biovolume with a precision of 5%.
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Heldal, M., Norland, S., & Tumyr, O. (1985) · Applied and Environmental Microbiology · doi:10.1128/aem.50.5.1251-1257.1985 · Cited by 150
Cited by
Zooplankton grazing and growth: Scaling within the 2‐2,‐μm body size range
Hansen, P. J., Bjørnsen, P. K., & Hansen, B. W. (1997) · Limnology and Oceanography · doi:10.4319/lo.1997.42.4.0687 · Cited by 764
Self-citationLoferer-Krößbacher, M., Klima, J., & Psenner, R. (1998) · Applied and Environmental Microbiology · doi:10.1128/aem.64.2.688-694.1998 · Cited by 406
Dominance of bacterial biomass in the Sargasso Sea and its ecological implications
Fuhrman, J., Sleeter, T., Carlson, C., & Proctor, L. (1989) · Marine Ecology Progress Series · doi:10.3354/meps057207 · Cited by 362
B�rsheim, K. Y., Bratbak, G., & Heldal, M. (1990) · Applied and Environmental Microbiology · doi:10.1128/aem.56.2.352-356.1990 · Cited by 318
2 Direct Methods and Biomass Estimation
Fry, J. C. (1990) · Methods in Microbiology · doi:10.1016/s0580-9517(08)70239-3 · Cited by 314
Metadata
- Authors
- Bjørnsen, P. K.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1986-06
- Volume
- 51
- Issue
- 6
- Pages
- 1199-1204
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1848325228
- Cited by
- 312
- References
- 1
- FWCI
- 11.45
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Bjørnsen, P. K. (1986). Automatic Determination of Bacterioplankton Biomass by Image Analysis. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.51.6.1199-1204.1986
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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