The DOI matches its Crossref record.
Specific and sensitive plate assay for bacterial lipases
Open accessAbstract
A plate assay to detect bacterial lipase (EC 3.1.1.3) in a medium containing trioleoylglycerol and the fluorescent dye rhodamine B is presented. Substrate hydrolysis causes the formation of orange fluorescent halos around bacterial colonies visible upon UV irradiation. The logarithm of lipase activity from cell-free culture supernatants is linearly correlated with the diameter of halos, thereby allowing quantitation of lipase activities ranging from 1 to 30 nkat.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Isolation and characterization of a thermophilic lipase from Bacillus thermoleovorans ID-1
Lee, D. (1999) · FEMS Microbiology Letters · doi:10.1016/s0378-1097(99)00440-1 · Cited by 301
Fluorogenic and chromogenic substrates used in bacterial diagnostics
Manafi, M., Kneifel, W., & Bascomb, S. (1991) · Microbiological Reviews · doi:10.1128/mmbr.55.3.335-348.1991 · Cited by 277
Hube, B., Stehr, F., Bossenz, M., Mazur, A., Kretschmar, M., & Schäfer, W. (2000) · Archives of Microbiology · doi:10.1007/s002030000218 · Cited by 212
Hernández, A., Martín, A., Aranda, E., Pérez-Nevado, F., & Córdoba, M. G. (2007) · Food Microbiology · doi:10.1016/j.fm.2006.07.022 · Cited by 155
Antagonism of Trichoderma species on Cladosporium herbarum and their enzimatic characterization
Barbosa, M. A. G., Rehn, K. G., Menezes, M., & Mariano, R. D. L. R. (2001) · Brazilian Journal of Microbiology · doi:10.1590/s1517-83822001000200005 · Cited by 80
Metadata
- Authors
- Kouker, G., & Jaeger, K. E.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1987-01
- Volume
- 53
- Issue
- 1
- Pages
- 211-213
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1762307288
- Cited by
- 748
- References
- 0
- FWCI
- 3.21
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Specific and sensitive plate assay for bacterial lipases. https://app.cochat.ai/papers/10.1128/aem.53.1.211-213.1987
- The paper
- Kouker, G., & Jaeger, K. E. (1987). Specific and sensitive plate assay for bacterial lipases. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.53.1.211-213.1987
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
