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Genetic engineering of ethanol production in Escherichia coli
Open accessAbstract
The genes encoding essential enzymes of the fermentative pathway for ethanol production in Zymomonas mobilis, an obligately ethanologenic bacterium, were inserted into Escherichia coli under the control of a common promoter. Alcohol dehydrogenase II and pyruvate decarboxylase from Z. mobilis were expressed at high levels in E. coli, resulting in increased cell growth and the production of ethanol as the principal fermentation product from glucose. These results demonstrate that it is possible to change the fermentation products of an organism, such as E. coli, by the addition of genes encoding appropriate enzymes which form an alternative system for the regeneration of NAD+.
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Cited by
Bio-ethanol – the fuel of tomorrow from the residues of today
Hahn-Hägerdal, B., Galbe, M., Gorwa-Grauslund, M. F., Lidén, G., & Zacchi, G. (2006) · Trends in Biotechnology · doi:10.1016/j.tibtech.2006.10.004 · Cited by 1,513
Fermentation of lignocellulosic hydrolysates for ethanol production
Olsson, L., & Hahn-Hägerdal, B. (1996) · Enzyme and Microbial Technology · doi:10.1016/0141-0229(95)00157-3 · Cited by 783
B IOMASS E THANOL: Technical Progress, Opportunities, and Commercial Challenges
Wyman, C. E. (1999) · Annual Review of Energy and the Environment · doi:10.1146/annurev.energy.24.1.189 · Cited by 601
Energy and Environmental Aspects of Using Corn Stover for Fuel Ethanol
Sheehan, J., Aden, A., Paustian, K., Killian, K., Brenner, J., Walsh, M., & Nelson, R. (2003) · Journal of Industrial Ecology · doi:10.1162/108819803323059433 · Cited by 567
Microdiesel: Escherichia coli engineered for fuel production
Kalscheuer, R., Stölting, T., & Steinbüchel, A. (2006) · Microbiology · doi:10.1099/mic.0.29028-0 · Cited by 490
Metadata
- Authors
- Ingram, L. O., Conway, T., Clark, D. P., Sewell, G. W., & Preston, J. F.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1987-10
- Volume
- 53
- Issue
- 10
- Pages
- 2420-2425
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1482017157
- Cited by
- 492
- References
- 0
- FWCI
- 1.85
- Type
- journal article
- Language
- en
- Open access
- green
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- Ingram, L. O., Conway, T., Clark, D. P., Sewell, G. W., & Preston, J. F. (1987). Genetic engineering of ethanol production in Escherichia coli. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.53.10.2420-2425.1987
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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