Properties of Alanine Dehydrogenase and Aspartase from Propionibacterium freudenreichii subsp. shermanii
Open accessAbstract
During lactate fermentation by Propionibacterium freudenreichii subsp. shermanii ATCC 9614, the only amino acid metabolized was aspartate. After lactate exhaustion, alanine was one of the two amino acids to be metabolized. For every 3 mol of alanine metabolized, 2 mol of propionate, 1 mol each of acetate and CO(2), and 3 mol of ammonia were formed. The specific activity of alanine dehydrogenase was 0.08 U/mg of protein during lactate fermentation, and it increased to 0.9 U/mg of protein after lactate exhaustion. Alanine dehydrogenase and aspartase, key enzymes in the metabolism of alanine and aspartate, respectively, were partially purified, and some of their properties were studied. Alanine dehydrogenase had a pH optimum of 9.2 to 9.6 and high K(m) values for both NAD (1 to 4 mM) and alanine (7 to 20 mM). Activity was inhibited by low concentrations of pyruvate and NADH. The pH optimum of aspartase decreased from approximately 7.5 to approximately 6.4 when the MgCl(2) and aspartate concentrations were decreased. Plots of aspartate concentration versus activity showed either hyperbolic or sigmoidal kinetics (interaction coefficient, up to a value of 3.1), depending on pH and MgCl(2) concentration. MgCl(2) was either an activator or an inhibitor, depending on pH and its concentration. Aspartase activity was inhibited by low concentrations of fumarate. The properties of alanine dehydrogenase and aspartase are consistent with the finding that aspartate is metabolized during lact…
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Asp, Ala and Ser are the most degraded amino acids by both resting cells and growing cells of PAB.
“They showed that Asp, Ala and Ser are the most degraded amino acids by both resting cells and growing cells of PAB.”
From Production of cheese flavour compounds derived from amino acid catabolism by <i>Propionibacterium freudenreichii</i> (2002) · cites this paper as [15]
Asp, Ala and Ser are the most degraded amino acids by both resting cells and growing cells of PAB.
“They showed that Asp, Ala and Ser are the most degraded amino acids by both resting cells and growing cells of PAB.”
From Production of cheese flavour compounds derived from amino acid catabolism by <i>Propionibacterium freudenreichii</i> (2002) · cites this paper as [16]
Two enzymes have been partially purified: aspartase and alanine dehydrogenase, respectively involved in the formation of fumarate from Asp and pyruvate from Ala.
“Two enzymes have been partially purified: aspartase and alanine dehydrogenase, respectively involved in the formation of fumarate from Asp and pyruvate from Ala [16].”
From Production of cheese flavour compounds derived from amino acid catabolism by <i>Propionibacterium freudenreichii</i> (2002) · cites this paper as [16]
The end products of these amino acid degradations are acetate, propionate and succinate (from Asp), in addition to carbon dioxide and/or ammonia.
“The end products of these amino acid degradations are acetate, propionate and succinate (from Asp), in addition to carbon dioxide and/or ammonia [11,15].”
From Production of cheese flavour compounds derived from amino acid catabolism by <i>Propionibacterium freudenreichii</i> (2002) · cites this paper as [15]
Cited by
Sorted by citations
Thierry, A., & Maillard, M.-B. (2002) · Le Lait · doi:10.1051/lait:2001002 · Cited by 110 · see citing works
- Authors
- Crow, V. L.
- Affiliations
- New Zealand Dairy Research Institute, Palmerston North, New Zealand
- Corresponding author
- Crow, Vaughan L.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1987-08
- Volume
- 53
- Issue
- 8
- Pages
- 1885-1892
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC204019
- OpenAlex
- W1553175189
- Cited by
- 26
- References
- 0
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2016: 1 · 2014: 1 · 2012: 1
- FWCI
- 0.65
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
- Keywords
- alanine dehydrogenase, aspartase, Propionibacterium freudenreichii subsp. shermanii, lactic acid fermentation, aspartate metabolism, alanine metabolism, enzyme kinetics, enzyme inhibition, magnesium chloride, NAD+, pyruvate, fumarate, propionate production, ammonia synthesis
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
