Cited by 2,395Microbial Fuel Cells and BioremediationMicrobial metabolism and enzyme functionMicrobial Community Ecology and Physiology

Novel Mode of Microbial Energy Metabolism: Organic Carbon Oxidation Coupled to Dissimilatory Reduction of Iron or Manganese

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Lovley, Derek R., Phillips, Elizabeth J. P.·1988·Applied and Environmental Microbiology

Abstract

A dissimilatory Fe(III)- and Mn(IV)-reducing microorganism was isolated from freshwater sediments of the Potomac River, Maryland. The isolate, designated GS-15, grew in defined anaerobic medium with acetate as the sole electron donor and Fe(III), Mn(IV), or nitrate as the sole electron acceptor. GS-15 oxidized acetate to carbon dioxide with the concomitant reduction of amorphic Fe(III) oxide to magnetite (Fe(3)O(4)). When Fe(III) citrate replaced amorphic Fe(III) oxide as the electron acceptor, GS-15 grew faster and reduced all of the added Fe(III) to Fe(II). GS-15 reduced a natural amorphic Fe(III) oxide but did not significantly reduce highly crystalline Fe(III) forms. Fe(III) was reduced optimally at pH 6.7 to 7 and at 30 to 35 degrees C. Ethanol, butyrate, and propionate could also serve as electron donors for Fe(III) reduction. A variety of other organic compounds and hydrogen could not. MnO(2) was completely reduced to Mn(II), which precipitated as rhodochrosite (MnCO(3)). Nitrate was reduced to ammonia. Oxygen could not serve as an electron acceptor, and it inhibited growth with the other electron acceptors. This is the first demonstration that microorganisms can completely oxidize organic compounds with Fe(III) or Mn(IV) as the sole electron acceptor and that oxidation of organic matter coupled to dissimilatory Fe(III) or Mn(IV) reduction can yield energy for microbial growth. GS-15 provides a model for how enzymatically catalyzed reactions can be quantitatively signi…

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Crossref · 10.1128/aem.54.6.1472-1480.1988

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Authors
Lovley, D. R., & Phillips, E. J. P.
Affiliations
United States Geological Survey (US)
Venue
Applied and Environmental Microbiology
Published
1988-06
Volume
54
Issue
6
Pages
1472-1480
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
OpenAlex
W2164513556
Cited by
2,395
References
2
Citations by year
2026: 70 · 2025: 62 · 2024: 69 · 2023: 77 · 2022: 74 · 2021: 78 · 2020: 73 · 2019: 111 · 2018: 113 · 2017: 112 · 2016: 97 · 2015: 120 · 2014: 112 · 2013: 97 · 2012: 112
FWCI
32.85
Type
journal article
Language
en
Open access
Locations
  • Applied and Environmental Microbiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • ScholarWorks@UMassAmherst (University of Massachusetts Amherst) (submittedVersion)
Topics
  • Physical Sciences › Environmental Science › Environmental Engineering › Microbial Fuel Cells and Bioremediation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
  • Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
Keywords
dissimilatory iron reduction, organic carbon oxidation, acetate oxidation, anaerobic microorganisms, Fe(III) oxide reduction, magnetite, rhodochrosite, nitrate reduction, electron donors, freshwater sediments, Potomac River, microbial energy metabolism

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