The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Construction of a lactococcal expression vector: expression of hen egg white lysozyme in Lactococcus lactis subsp. lactis
Open accessAbstract
A pair of vectors for expression of heterologous genes in Lactococcus lactis was constructed. In addition to an origin of replication that has a broad host range, these vectors contain a multiple cloning site flanked by gene expression signals originating from L. lactis subsp. cremoris Wg2. The two vectors, about 3.7 kilobase pairs in size, differ only in the type of antibiotic resistance they confer to their hosts. pMG36 carries a kanamycin resistance marker, which was replaced by an erythromycin resistance marker in pMG36e. As an example of the use of these vectors, the hen egg white lysozyme-coding sequence was inserted. A fusion protein of the expected size was detected in a transformed L. lactis subsp. lactis strain by using Western blotting (immunoblotting).
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Cites
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Cited by
Piard, J. C., & Desmazeaud, M. (1992) · Le Lait · doi:10.1051/lait:199229 · Cited by 266
Cogan, T. M., & Hill, C. (1993) · Cheese: Chemistry, Physics and Microbiology · doi:10.1007/978-1-4615-2650-6_6 · Cited by 139
Metadata
- Authors
- van de Guchte, M., van der Vossen, J. M., Kok, J., & Venema, G.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1989-01
- Volume
- 55
- Issue
- 1
- Pages
- 224-228
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1509011059
- Cited by
- 295
- References
- 1
- FWCI
- 4.19
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: Construction of a lactococcal expression vector: expression of hen egg white lysozyme in Lactococcus lactis subsp. lactis. https://app.cochat.ai/papers/10.1128/aem.55.1.224-228.1989
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- van de Guchte, M., van der Vossen, J. M., Kok, J., & Venema, G. (1989). Construction of a lactococcal expression vector: expression of hen egg white lysozyme in Lactococcus lactis subsp. lactis. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.55.1.224-228.1989
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
