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Genetic improvement of Escherichia coli for ethanol production: chromosomal integration of Zymomonas mobilis genes encoding pyruvate decarboxylase and alcohol dehydrogenase II
Open accessAbstract
Zymomonas mobilis genes for pyruvate decarboxylase (pdc) and alcohol dehydrogenase II (adhB) were integrated into the Escherichia coli chromosome within or near the pyruvate formate-lyase gene (pfl). Integration improved the stability of the Z. mobilis genes in E. coli, but further selection was required to increase expression. Spontaneous mutants were selected for resistance to high level of chloramphenicol that also expressed high levels of the Z. mobilis genes. Analogous mutants were selected for increased expression of alcohol dehydrogenase on aldehyde indicator plates. These mutants were functionally equivalent to the previous plasmid-based strains for the fermentation of xylose and glucose to ethanol. Ethanol concentrations of 54.4 and 41.6 g/liter were obtained from 10% glucose and 8% xylose, respectively. The efficiency of conversion exceeded theoretical limits (0.51 g of ethanol/g of sugar) on the basis of added sugars because of the additional production of ethanol from the catabolism of complex nutrients. Further mutations were introduced to inactivate succinate production (frd) and to block homologous recombination (recA).
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Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
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Sauer, U., Lasko, D. R., Fiaux, J., Hochuli, M., Glaser, R., Szyperski, T., Wüthrich, K., & Bailey, J. E. (1999) · Journal of Bacteriology · doi:10.1128/jb.181.21.6679-6688.1999 · Cited by 371
Metadata
- Authors
- Ohta, K., Beall, D. S., Mejia, J. P., Shanmugam, K. T., & Ingram, L. O.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1991-04
- Volume
- 57
- Issue
- 4
- Pages
- 893-900
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1590308370
- Cited by
- 488
- References
- 1
- FWCI
- 3.17
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Genetic improvement of Escherichia coli for ethanol production: chromosomal integration of Zymomonas mobilis genes encoding pyruvate decarboxylase and alcohol dehydrogenase II. https://app.cochat.ai/papers/10.1128/aem.57.4.893-900.1991
- The paper
- Ohta, K., Beall, D. S., Mejia, J. P., Shanmugam, K. T., & Ingram, L. O. (1991). Genetic improvement of Escherichia coli for ethanol production: chromosomal integration of Zymomonas mobilis genes encoding pyruvate decarboxylase and alcohol dehydrogenase II. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.57.4.893-900.1991
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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