The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Pseudomonas putida KT2442 cultivated on glucose accumulates poly(3-hydroxyalkanoates) consisting of saturated and unsaturated monomers
Open accessAbstract
The biosynthesis of poly(3-hydroxyalkanoates) (PHAs) by Pseudomonas putida KT2442 during growth on carbohydrates was studied. PHAs isolated from P. putida cultivated on glucose, fructose, and glycerol were found to have a very similar monomer composition. In addition to the major constituent 3-hydroxydecanoate, six other monomers were found to be present: 3-hydroxyhexanoate, 3-hydroxyoctanoate, 3-hydroxydodecanoate, 3-hydroxydodecenoate, 3-hydroxytetradecanoate, and 3-hydroxytetradecenoate. The identity of all seven 3-hydroxy fatty acids was established by gas chromatography-mass spectrometry, one-dimensional 1H-nuclear magnetic resonance, and two-dimensional double-quantum filtered correlation spectroscopy 1H-nuclear magnetic resonance. The chemical structures of the monomer units are identical to the structure of the acyl moiety of the 3-hydroxyacyl-acyl carrier protein intermediates of de novo fatty acid biosynthesis. Furthermore, the degree of unsaturation of PHA and membrane lipids is similarly influenced by shifts in the cultivation temperature. These results strongly indicate that, during growth on nonrelated substrates, PHA monomers are derived from intermediates of de novo fatty acid biosynthesis. Analysis of a P. putida pha mutant and complementation of this mutant with the cloned pha locus revealed that the PHA polymerase genes necessary for PHA synthesis from octanoate are also responsible for PHA formation from glucose.
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Cites
Escherichia Coli and Salmonella: Typhimurium Cellular and Molecular Biology
Neidhardt, F. C., & Ingraham, J. L. (1987) · Medical Entomology and Zoology · Cited by 4,089
Bagdasarian, M., Lurz, R., Rückert, B., Franklin, F. C. H., Bagdasarian, M. M., Frey, J., & Timmis, K. N. (1981) · Gene · doi:10.1016/0378-1119(81)90080-9 · Cited by 1,106
Brandl, H., Gross, R. A., Lenz, R. W., & Fuller, R. C. (1988) · Applied and Environmental Microbiology · doi:10.1128/aem.54.8.1977-1982.1988 · Cited by 869
Friedman, A. M., Long, S. R., Brown, S. E., Buikema, W. J., & Ausubel, F. M. (1982) · Gene · doi:10.1016/0378-1119(82)90167-6 · Cited by 827
Lageveen, R. G., Huisman, G. W., Preusting, H., Ketelaar, P., Eggink, G., & Witholt, B. (1988) · Applied and Environmental Microbiology · doi:10.1128/aem.54.12.2924-2932.1988 · Cited by 694
Self-citationSchubert, P., Steinbüchel, A., & Schlegel, H. G. (1988) · Journal of Bacteriology · doi:10.1128/jb.170.12.5837-5847.1988 · Cited by 523
Poly-β-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16
Peoples, O. P., & Sinskey, A. J. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)84825-1 · Cited by 473
de Smet, M. J., Eggink, G., Witholt, B., Kingma, J., & Wynberg, H. (1983) · Journal of Bacteriology · doi:10.1128/jb.154.2.870-878.1983 · Cited by 355
Self-citationTimm, A., & Steinbüchel, A. (1990) · Applied and Environmental Microbiology · doi:10.1128/aem.56.11.3360-3367.1990 · Cited by 342
Gross, R. A., DeMello, C., Lenz, R. W., Brandl, H., & Fuller, R. C. (1989) · Macromolecules · doi:10.1021/ma00193a018 · Cited by 294
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Anjum, A., Zuber, M., Zia, K. M., Noreen, A., Anjum, M. N., & Tabasum, S. (2016) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2016.04.069 · Cited by 665
Poirier, Y., Nawrath, C., & Somerville, C. (1995) · Nature Biotechnology · doi:10.1038/nbt0295-142 · Cited by 480
Steinbüchel, A., & Lütke-Eversloh, T. (2003) · Biochemical Engineering Journal · doi:10.1016/s1369-703x(03)00036-6 · Cited by 470
A genome-scale metabolic reconstruction of Pseudomonas putida KT2440: i JN746 as a cell factory
Nogales, J., Palsson, B. Ø., & Thiele, I. (2008) · BMC Systems Biology · doi:10.1186/1752-0509-2-79 · Cited by 261
Metadata
- Authors
- Huijberts, G. N., Eggink, G., de Waard, P., Huisman, G. W., & Witholt, B.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1992-02
- Volume
- 58
- Issue
- 2
- Pages
- 536-544
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1760672256
- Cited by
- 314
- References
- 19
- FWCI
- 14.10
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Huijberts, G. N., Eggink, G., de Waard, P., Huisman, G. W., & Witholt, B. (1992). Pseudomonas putida KT2442 cultivated on glucose accumulates poly(3-hydroxyalkanoates) consisting of saturated and unsaturated monomers. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.58.2.536-544.1992
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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