The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Detection of low numbers of bacterial cells in soils and sediments by polymerase chain reaction
Open accessAbstract
Polymerase chain reaction was used to amplify the low copy number of two 16S ribosomal gene fragments from soil and sediment extracts. Total DNA for polymerase chain reaction was extracted from 1 g of seeded or unseeded samples by a rapid freeze-and-thaw method. Amplified DNA fragments can be detected in DNA fractions isolated from seeded soil containing less than 3 Escherichia coli cells and from seeded sediments containing less than 10 cells. This research demonstrated that coupling polymerase chain reaction to direct DNA extraction improves sensitivity by 1 and 2 orders of magnitude for sediments and soils, respectively. This technique could become a powerful tool for genetic ecology studies.
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Cites
Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985) · Science · doi:10.1126/science.2999980 · Cited by 8,956
Cited by
Towards a minimum data set to assess soil organic matter quality in agricultural soils
Gregorich, E. G., Carter, M. R., Angers, D. A., Monreal, C. M., & Ellert, B. H. (1994) · Canadian Journal of Soil Science · doi:10.4141/cjss94-051 · Cited by 1,150
Daniel, R. (2005) · Nature Reviews Microbiology · doi:10.1038/nrmicro1160 · Cited by 862
A strategy for optimizing quality and quantity of DNA extracted from soil
Bürgmann, H., Pesaro, M., Widmer, F., & Zeyer, J. (2001) · Journal of Microbiological Methods · doi:10.1016/s0167-7012(01)00213-5 · Cited by 317
Amplification of DNA from native populations of soil bacteria by using the polymerase chain reaction
Bruce, K. D., Hiorns, W. D., Hobman, J. L., Osborn, A. M., Strike, P., & Ritchie, D. A. (1992) · Applied and Environmental Microbiology · doi:10.1128/aem.58.10.3413-3416.1992 · Cited by 253
Metadata
- Authors
- Tsai, Y. L., & Olson, B. H.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1992-02
- Volume
- 58
- Issue
- 2
- Pages
- 754-757
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1511469905
- Cited by
- 359
- References
- 1
- FWCI
- 43.62
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Tsai, Y. L., & Olson, B. H. (1992). Detection of low numbers of bacterial cells in soils and sediments by polymerase chain reaction. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.58.2.754-757.1992
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
