Minimal Requirements for Exponential Growth of Lactococcus lactis
Open accessAbstract
A minimal growth medium containing glucose, acetate, vitamins, and eight amino acids allowed for growth of Lactococcus lactis subsp. lactis , with a specific growth rate in batch culture of μ = 0.3 h -1 . With 19 amino acids added, the growth rate increased to μ = 0.7 h -1 and the exponential growth phase proceeded until high cell concentrations were reached. We show that morpholinepropanesulfonic acid (MOPS) is a suitable buffer for L. lactis and may be applied in high concentrations.
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Strains of L. lactis are typically auxotrophic for several amino acids and need an extracellular protease to grow to high cell densities in milk.
“Strains of L. lactis are typically auxotrophic for several amino acids and in order to grow to high cell densities in milk they need an extracellular protease.”
From Modeled Structure of the Cell Envelope Proteinase of Lactococcus lactis (2020) · cites this paper as Jensen and Hammer, 1993
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- Authors
- Jensen, P. R., & Hammer, K.
- Affiliations
- The Netherlands Cancer Institute (NL)
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1993-12
- Volume
- 59
- Issue
- 12
- Pages
- 4363-4366
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC195913
- OpenAlex
- W1551703085
- Cited by
- 318
- References
- 0
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 6 · 2023: 3 · 2022: 2 · 2021: 11 · 2020: 12 · 2019: 6 · 2018: 6 · 2017: 16 · 2016: 9 · 2015: 11 · 2014: 15 · 2013: 11 · 2012: 12
- FWCI
- 13.65
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Technical University of Denmark, DTU Orbit (Technical University of Denmark, DTU) (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Lactococcus lactis, exponential growth, specific growth rate, amino acids, glucose, acetate, vitamins, MOPS buffer, batch culture, lactic acid bacteria
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