The DOI matches its Crossref record.
Use of rRNA fluorescence in situ hybridization for measuring the activity of single cells in young and established biofilms
Open accessAbstract
We describe the in situ use of rRNA-targeted fluorescent hybridization probes in combination with digital microscopy to quantify the cellular content of ribosomes in relationship to the growth rate of single cells of a specific population of sulfate-reducing bacteria in multispecies anaerobic biofilms. Using this technique, we inferred that this population was growing with an average generation time of 35 h in a young biofilm, whereas the doubling time in an established biofilm was significantly longer. Conventional chemical determinations of the RNA, DNA, and protein contents of this culture at different growth rates were also carried out, and the resulting data were compared with the rRNA fluorescence in situ hybridization data.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Blazewicz, S. J., Barnard, R. L., Daly, R. A., & Firestone, M. K. (2013) · The ISME Journal · doi:10.1038/ismej.2013.102 · Cited by 878
Microbiological Safety of Drinking Water
Szewzyk, U., Szewzyk, R., Manz, W., & Schleifer, K.-H. (2000) · Annual Review of Microbiology · doi:10.1146/annurev.micro.54.1.81 · Cited by 580
Tracking heavy water (D 2 O) incorporation for identifying and sorting active microbial cells
Berry, D., Mader, E., Lee, T. K., Woebken, D., Wang, Y., Zhu, D., Palatinszky, M., Schintlmeister, A., Schmid, M. C., Hanson, B. T., et al. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1420406112 · Cited by 455
Investigation of ciprofloxacin penetration into Pseudomonas aeruginosa biofilms
Suci, P. A., Mittelman, M. W., Yu, F. P., & Geesey, G. G. (1994) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.38.9.2125 · Cited by 357
Farookhi, H., & Xia, X. (2024) · Microorganisms · doi:10.3390/microorganisms12040768 · Cited by 7
Metadata
- Authors
- Poulsen, L. K., Ballard, G., & Stahl, D. A.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1993-05
- Volume
- 59
- Issue
- 5
- Pages
- 1354-1360
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1533811233
- Cited by
- 533
- References
- 0
- FWCI
- 43.78
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Use of rRNA fluorescence in situ hybridization for measuring the activity of single cells in young and established biofilms. https://app.cochat.ai/papers/10.1128/aem.59.5.1354-1360.1993
- The paper
- Poulsen, L. K., Ballard, G., & Stahl, D. A. (1993). Use of rRNA fluorescence in situ hybridization for measuring the activity of single cells in young and established biofilms. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.59.5.1354-1360.1993
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
