Cited by 94biodegradable polymer synthesis and propertiesBiopolymer Synthesis and ApplicationsMicrobial bioremediation and biosurfactants

The DOI matches its Crossref record.

Influence of Ammonium Salts and Cane Molasses on Growth of Alcaligenes eutrophus and Production of Polyhydroxybutyrate

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Beaulieu, M, Beaulieu, Y, Melinard, J, Pandian, S, Goulet, J·1995·Applied and Environmental Microbiology

Abstract

The production of polyhydroxybutyrate (PHB) by Alcaligenes eutrophus DSM 545 was studied in a synthetic medium with 3% glucose at pH 7.0 supplemented with several ammonium substrates and cane molasses. Growth was measured by dry cell weight, and the PHB content was measured by gas chromatography. The effects of ammonium sources such as sulfate, nitrate, phosphate, and chloride salts and those of different ammonium sulfate concentrations were evaluated. The best growth and PHB production were obtained with ammonium sulfate; however, NH(inf4)(sup+) concentrations between 0.5 and 1.5 g/liter showed no significant difference. Ammonium sulfate was therefore used as the sole source of NH(inf4)(sup+) for experiments with cane molasses as the growth activator. Optimal growth and PHB production were obtained with 0.3% molasses. However, the yields of biomass (39 to 48%) and PHB (17 to 26%) varied significantly among the different ammonium substrates and cane molasses concentrations.

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Crossref · 10.1128/aem.61.1.165-169.1995

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Metadata

Authors
Beaulieu, M., Beaulieu, Y., Melinard, J., Pandian, S., & Goulet, J.
Venue
Applied and Environmental Microbiology
Published
1995-01
Volume
61
Issue
1
Pages
165-169
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
OpenAlex
W2146978887
Cited by
94
References
0
Citations by year
2026: 1 · 2025: 3 · 2024: 4 · 2023: 5 · 2022: 2 · 2021: 3 · 2020: 8 · 2019: 6 · 2018: 2 · 2017: 2 · 2016: 6 · 2015: 4 · 2014: 5 · 2013: 3 · 2012: 7
FWCI
0.70
Type
journal article
Language
en
Open access
Locations
  • Applied and Environmental Microbiology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
  • Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Biopolymer Synthesis and Applications
  • Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
Keywords
polyhydroxybutyrate, Cupriavidus necator, ammonium salts, sugarcane molasses, ammonium sulfate, bacterial growth, PHB production, nitrogen source, glucose fermentation, biomass yield

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