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An apparent anomaly in the calculation of ash-free dry weights for the determination of cellular yields
Open accessAbstract
The use of ash-free dry weights results in a determination of CHON-containing cell mass that is 5 to 6% less than that determined on the basis of total cellular composition. This discrepancy derives from the fact that phosphorus and sulfur contribute greater weight to ash as oxides and salts of P and S than the contribution they make as part of the cellular fabric. In addition, in the absence of P and S, the unit carbon formula for this mass becomes changed, on the basis of an equivalent number of available electrons per unit of carbon formula weight.
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“In the experiments carried out by Battley (2013, 2007, 1995) [32], [35], [36] related to the Saccharomyces cerevisiae, he has established C6.33H10.21O3.53N as the empirical chemical formula for this yeast.”
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Araujo Granda, P., Gras, A., & Ginovart, M. (2016) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2016.08.001 · Cited by 9
Metadata
- Authors
- Battley, E. H.
- Corresponding author
- Battley, E H
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1995-04
- Volume
- 61
- Issue
- 4
- Pages
- 1655-1657
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC1388426
- OpenAlex
- W2128333452
- Cited by
- 23
- References
- 0
- Citations by year
- 2022: 1 · 2020: 1 · 2019: 1 · 2016: 2 · 2014: 1 · 2013: 2 · 2012: 1
- FWCI
- 0.31
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Keywords
- ash-free dry weight, cell yield, phosphorus, sulfur, ash content, microbial biomass
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How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: An apparent anomaly in the calculation of ash-free dry weights for the determination of cellular yields. https://app.cochat.ai/papers/10.1128/aem.61.4.1655-1657.1995
- The paper
- Battley, E. H. (1995). An apparent anomaly in the calculation of ash-free dry weights for the determination of cellular yields. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.61.4.1655-1657.1995
