The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Detection of mRNA by Reverse Transcription-PCR as an Indicator of Viability in Escherichia coli Cells
Open accessAbstract
The relationship between the detection of mRNA and cellular viability in Escherichia coli was investigated in cells killed by heat or ethanol. Reverse transcription-PCR (RT-PCR) methods were developed for detecting mRNA from rpoH , groEL , and tufA genes. mRNA from all three genes was detected immediately after the cells had been killed by heat or ethanol but gradually disappeared with time when dead cells were held at room temperature. In heat-killed cells, some mRNA targets became undetectable after 2 to 16 h, whereas after ethanol treatment, mRNA was still detected after 16 h. In contrast, 16S rRNA was detected by RT-PCR in all samples containing dead cells and did not disappear during a subsequent incubation of 16 h at room temperature. Of the different types of nucleic acid, mRNA is the most promising candidate for an indicator of viability in bacteria, but its persistence in dead cells depends on the inactivating treatment and subsequent holding conditions.
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Cites
Instability and decay of the primary structure of DNA
Lindahl, T. (1993) · Nature · doi:10.1038/362709a0 · Cited by 5,679
Cited by
Fungi in freshwaters: ecology, physiology and biochemical potential
Krauss, G.-J., Solé, M., Krauss, G., Schlosser, D., Wesenberg, D., & Bärlocher, F. (2011) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2011.00266.x · Cited by 340
Metadata
- Authors
- Sheridan, G. E. C., Masters, C. I., Shallcross, J. A., & Mackey, B. M.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1998-04
- Volume
- 64
- Issue
- 4
- Pages
- 1313-1318
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1852890074
- Cited by
- 382
- References
- 1
- FWCI
- 3.60
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Detection of mRNA by Reverse Transcription-PCR as an Indicator of Viability in Escherichia coli Cells. https://app.cochat.ai/papers/10.1128/aem.64.4.1313-1318.1998
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- Sheridan, G. E. C., Masters, C. I., Shallcross, J. A., & Mackey, B. M. (1998). Detection of mRNA by Reverse Transcription-PCR as an Indicator of Viability in Escherichia coli Cells. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.64.4.1313-1318.1998
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
