A Double-Selective Tissue Culture System for Isolation of Wild-Type Poliovirus from Sewage Applied in a Long-Term Environmental Surveillance
Open accessAbstract
We describe a simple, cost-efficient, double-selective method for isolation of wild-type poliovirus from sewage samples containing vaccine polioviruses and other enteroviruses, with a detection limit of 18 to 50 PFU per 1 to 2 liters of sewage. By this method we were able to process 1,700 sewage samples collected between 1991 and 1996, from which 10,472 plaques were isolated, 41 of them being identified as wild-type polioviruses.
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In suspected high-risk areas lacking effective AFP surveillance, supplementary surveillance activities, such as sampling community contacts of AFP cases, stool surveys of healthy children, or environmental sampling, have been implemented to increase sensitivity for detecting wild polioviruses.
“In suspected high-risk areas lacking effective AFP surveillance, supplementary surveillance activities, such as sampling community contacts of AFP cases, stool surveys of healthy children, or environmental sampling, have been implemented to increase sensitivity for detecting wild polioviruses (39, 48, 116, 131, 155, 190, 197).”
From VACCINE-DERIVED POLIOVIRUSES AND THE ENDGAME STRATEGY FOR GLOBAL POLIO ERADICATION (2005) · cites this paper as [116]
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References 24 · Cited by 39
- Authors
- Manor, Y., Handsher, R., Halmut, T., Neuman, M., Abramovitz, B., Mates, A., & Mendelson, E.
- Affiliations
- Sheba Medical Center (IL)
- Israel Ministry of Health (IL)
- ORCID
- Mendelson, E.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1999-04
- Volume
- 65
- Issue
- 4
- Pages
- 1794-1797
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC91255
- OpenAlex
- W1539936102
- Cited by
- 39
- References
- 24
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 2 · 2022: 1 · 2020: 2 · 2017: 2 · 2016: 1 · 2015: 1 · 2014: 7 · 2012: 2
- FWCI
- 1.21
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Viral Infections and Immunology Research
- Physical Sciences › Environmental Science › Water Science and Technology › Fecal contamination and water quality
- Health Sciences › Medicine › Infectious Diseases › Viral gastroenteritis research and epidemiology
- Keywords
- wild-type poliovirus, wastewater, virus isolation, environmental surveillance, enterovirus, detection limit
- MeSH terms
- Animals, Chlorocebus aethiops, Enterovirus, Environmental Monitoring, Humans, Neutralization Tests, Viral Plaque Assay, Poliomyelitis, Poliovirus Vaccine, Inactivated, Sewage, Tumor Cells, Cultured, Virology, Poliovirus
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