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Export of Virulence Genes and Shiga Toxin by Membrane Vesicles of Escherichia coli O157:H7
Open accessAbstract
Membrane vesicles released by Escherichia coli O157:H7 into culture medium were purified and analyzed for protein and DNA content. Electron micrographs revealed vesicles that are spherical, range in size from 20 to 100 nm, and have a complete bilayer. Analysis of vesicle protein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis demonstrates vesicles that contain many proteins with molecular sizes similar to outer membrane proteins and a number of cellular proteins. Immunoblot (Western) analysis of vesicles suggests the presence of cell antigens. Treatment of vesicles with exogenous DNase hydrolyzed surface-associated DNA; PCR demonstrated that vesicles contain DNA encoding the virulence genes eae , stx1 and stx2 , and uidA , which encodes for β-galactosidase. Immunoblot analysis of intact and lysed, proteinase K-treated vesicles demonstrate that Shiga toxins 1 and 2 are contained within vesicles. These results suggest that vesicles contain toxic material and transfer experiments demonstrate that vesicles can deliver genetic material to other gram-negative organisms.
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Cited by
Biological Functions and Biogenesis of Secreted Bacterial Outer Membrane Vesicles
Kulp, A., & Kuehn, M. J. (2010) · Annual Review of Microbiology · doi:10.1146/annurev.micro.091208.073413 · Cited by 1,533
Proteomics in gram‐negative bacterial outer membrane vesicles
Lee, E., Choi, D., Kim, K., & Gho, Y. S. (2008) · Mass Spectrometry Reviews · doi:10.1002/mas.20175 · Cited by 339
Al‐Nedawi, K., Mian, M. F., Hossain, N., Karimi, K., Mao, Y., Forsythe, P., Min, K. K., Stanisz, A. M., Kunze, W. A., & Bienenstock, J. (2015) · The FASEB Journal · doi:10.1096/fj.14-259721 · Cited by 177
Functional Advantages of Porphyromonas gingivalis Vesicles
Ho, M.-H., Chen, C.-H., Goodwin, J. S., Wang, B.-Y., & Xie, H. (2015) · PLOS ONE · doi:10.1371/journal.pone.0123448 · Cited by 167
Metadata
- Authors
- Kolling, G. L., & Matthews, K. R.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1999-05
- Volume
- 65
- Issue
- 5
- Pages
- 1843-1848
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2115765556
- Cited by
- 320
- References
- 0
- FWCI
- 2.56
- Type
- journal article
- Language
- en
- Open access
- green
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- Kolling, G. L., & Matthews, K. R. (1999). Export of Virulence Genes and Shiga Toxin by Membrane Vesicles of Escherichia coli O157:H7. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.65.5.1843-1848.1999
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