The DOI matches its Crossref record.
Rapid Method for Coextraction of DNA and RNA from Natural Environments for Analysis of Ribosomal DNA- and rRNA-Based Microbial Community Composition
Open accessAbstract
A rapid protocol for the extraction of total nucleic acids from environmental samples is described. The method facilitates concomitant assessment of microbial 16S rRNA diversity by PCR and reverse transcription-PCR amplification from a single extraction. Denaturing gradient gel electrophoresis microbial community analysis differentiated the active component (rRNA derived) from the total bacterial diversity (ribosomal DNA derived) down the horizons of an established grassland soil.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Archaea predominate among ammonia-oxidizing prokaryotes in soils
Leininger, S., Urich, T., Schloter, M., Schwark, L., Qi, J., Nicol, G. W., Prosser, J. I., Schuster, S. C., & Schleper, C. (2006) · Nature · doi:10.1038/nature04983 · Cited by 2,497
Tourna, M., Freitag, T. E., Nicol, G. W., & Prosser, J. I. (2008) · Environmental Microbiology · doi:10.1111/j.1462-2920.2007.01563.x · Cited by 773
Anderson, C. R., Condron, L. M., Clough, T. J., Fiers, M., Stewart, A., Hill, R. A., & Sherlock, R. R. (2011) · Pedobiologia · doi:10.1016/j.pedobi.2011.07.005 · Cited by 768
Dempster, D. N., Gleeson, D. B., Solaiman, Z. M., Jones, D. L., & Murphy, D. V. (2012) · Plant and Soil · doi:10.1007/s11104-011-1067-5 · Cited by 509
Angelberger, S., Reinisch, W., Makristathis, A., Lichtenberger, C., Dejaco, C., Papay, P., Novacek, G., Trauner, M., Loy, A., & Berry, D. (2013) · American Journal of Gastroenterology · doi:10.1038/ajg.2013.257 · Cited by 330
Metadata
- Authors
- Griffiths, R. I., Whiteley, A. S., O'Donnell, A. G., & Bailey, M. J.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2000-12
- Volume
- 66
- Issue
- 12
- Pages
- 5488-5491
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2123088611
- Cited by
- 1,676
- References
- 0
- FWCI
- 16.49
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Rapid Method for Coextraction of DNA and RNA from Natural Environments for Analysis of Ribosomal DNA- and rRNA-Based Microbial Community Composition. https://app.cochat.ai/papers/10.1128/aem.66.12.5488-5491.2000
- The paper
- Griffiths, R. I., Whiteley, A. S., O'Donnell, A. G., & Bailey, M. J. (2000). Rapid Method for Coextraction of DNA and RNA from Natural Environments for Analysis of Ribosomal DNA- and rRNA-Based Microbial Community Composition. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.66.12.5488-5491.2000
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
