The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.
Overexpression of Trigger Factor Prevents Aggregation of Recombinant Proteins in Escherichia coli
Open accessAbstract
To examine the effects of overexpression of trigger factor (TF) on recombinant proteins produced in Escherichia coli , we constructed plasmids that permitted controlled expression of TF alone or together with the GroEL-GroES chaperones. The following three proteins that are prone to aggregation were tested as targets: mouse endostatin, human oxygen-regulated protein ORP150, and human lysozyme. The results revealed that TF overexpression had marked effects on the production of these proteins in soluble forms, presumably through facilitating correct folding. Whereas overexpression of TF alone was sufficient to prevent aggregation of endostatin, overexpression of TF together with GroEL-GroES was more effective for ORP150 and lysozyme, suggesting that TF and GroEL-GroES play synergistic roles in vivo. Although coexpression of the DnaK-DnaJ-GrpE chaperones was also effective for endostatin and ORP150, coexpression of TF and GroEL-GroES was more effective for lysozyme. These results attest to the usefulness of the present expression plasmids for improving protein production in E. coli .
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Cites
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Self-citationPolypeptide Flux through Bacterial Hsp70
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A ribosome-associated peptidyl-prolyl cis/trans isomerase identified as the trigger factor.
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Cited by
Recombinant protein expression in Escherichia coli: advances and challenges
Rosano, G. L., & Ceccarelli, E. A. (2014) · Frontiers in Microbiology · doi:10.3389/fmicb.2014.00172 · Cited by 2,556
Protein production and purification
Structural Genomics Consortium, Biologiques, A. E. F. D. M., Berkeley Structural Genomics Center, China Structural Genomics Consortium, Integrated Center for Structure and Function Innovation, Israel Structural Proteomics Center, Joint Center for Structural Genomics, Midwest Center for Structural Genomics, New York Structural GenomiX Research Center for Structural Genomics, Northeast Structural Genomics Consortium, et al. (2008) · Nature Methods · doi:10.1038/nmeth.f.202 · Cited by 916
Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli
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Gross, S., Cairns, R. A., Minden, M. D., Driggers, E. M., Bittinger, M. A., Jang, H. G., Sasaki, M., Jin, S., Schenkein, D. P., Su, S. M., et al. (2010) · Journal of Experimental Medicine · doi:10.1084/jem.20092506 · Cited by 736
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Metadata
- Authors
- Nishihara, K., Kanemori, M., Yanagi, H., & Yura, T.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2000-03
- Volume
- 66
- Issue
- 3
- Pages
- 884-889
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2154106340
- Cited by
- 304
- References
- 17
- FWCI
- 2.82
- Type
- journal article
- Language
- en
- Open access
- green
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