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Bovine Rotavirus Nonstructural Protein 4 Produced by Lactococcus lactis Is Antigenic and Immunogenic
Open accessAbstract
Rotavirus nonstructural protein 4 (NSP4) can induce diarrhea in mice. To get insight into the biological effects of NSP4, production of large quantities of this protein is necessary. We first tried to produce the protein in Escherichia coli, but the nsp4 gene proved to be unstable. The capacity of the generally regarded as safe organism Lactococcus lactis to produce NSP4 either intra- or extracellularly was then investigated by using the nisin-controlled expression system. Production of recombinant NSP4 (rNSP4) was observed in L. lactis for both locations. In spite of a very low secretion efficiency, the highest level of production was obtained with the fusion between a lactococcal signal peptide and rNSP4. Cultures of the rNSP4-secreting strain were injected into rabbits, and a specific immune response was elicited. The anti-rNSP4 antibodies produced in these rabbits recognized NSP4 in MA104 cells infected by rotavirus. We showed that L. lactis is able to produce antigenic and immunogenic rNSP4 and thus is a good organism for producing viral antigens.
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“As a noncolonizing bacterium that is nevertheless able to survive in the gastrointestinal tract, it constitutes an appropriate vehicle for the delivery of antigens in the gastrointestinal tract for mucosal immunization (4, 7, 25, 30, 37).”
From Determination of the Domain of the Lactobacillus delbrueckii subsp. bulgaricus Cell Surface Proteinase PrtB Involved in Attachment to the Cell Wall after Heterologous Expression of the prtB Gene in Lactococcus lactis (2003) · cites this paper as [7]
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References 31 · Cited by 104
Research works printed but not linked: 1
- Kapikian A. Z. Chanock R. M. Rotaviruses Fields virology 3rd ed. Fields B. N. Knipe D. M. Howley P. M. 1996 1657 1708 Lippincott-Raven Press New York N.Y
Metadata
- Authors
- Enouf, V., Langella, P., Commissaire, J., Cohen, J., & Corthier, G.
- Affiliations
- Virologie et Immunologie Moléculaires (FR)
- Unité de Recherche Technologie et Analyses Laitières (FR)
- <!--label omitted: 3-->Unitéd'Ecologie et de Physiologie du Tube Digestif,3INRA, 78352 Jouy en Josas, France
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2001-04
- Volume
- 67
- Issue
- 4
- Pages
- 1423-1428
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC92750
- OpenAlex
- W2039604184
- Cited by
- 104
- References
- 31
- Citations by year
- 2024: 1 · 2023: 1 · 2021: 3 · 2020: 1 · 2019: 1 · 2018: 3 · 2017: 1 · 2016: 3 · 2015: 5 · 2014: 5 · 2013: 3 · 2012: 5
- FWCI
- 17.09
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Medicine › Infectious Diseases › Viral gastroenteritis research and epidemiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- bovine rotavirus, NSP4, Lactococcus lactis, recombinant protein expression, signal peptide fusion, antigenicity, immunogenicity, rotavirus gastroenteritis, viral antigen production, MA104 cells
- MeSH terms
- Animals, Antibodies, Viral, Antigens, Viral, Cattle, Escherichia coli, Glycoproteins, Immunization, Rabbits, Recombinant Fusion Proteins, Rotavirus, Lactococcus lactis, Toxins, Biological, Viral Nonstructural Proteins
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