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DNA Extraction from Soils: Old Bias for New Microbial Diversity Analysis Methods
Open accessAbstract
The impact of three different soil DNA extraction methods on bacterial diversity was evaluated using PCR-based 16S ribosomal DNA analysis. DNA extracted directly from three soils showing contrasting physicochemical properties was subjected to amplified ribosomal DNA restriction analysis and ribosomal intergenic spacer analysis (RISA). The obtained RISA patterns revealed clearly that both the phylotype abundance and the composition of the indigenous bacterial community are dependent on the DNA recovery method used. In addition, this effect was also shown in the context of an experimental study aiming to estimate the impact on soil biodiversity of the application of farmyard manure or sewage sludge onto a monoculture of maize for 15 years.
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Cited by
Smith, C. J., & Osborn, A. M. (2009) · FEMS Microbiology Ecology · doi:10.1111/j.1574-6941.2008.00629.x · Cited by 937
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Lindahl, B. D., Nilsson, R. H., Tedersoo, L., Abarenkov, K., Carlsen, T., Kjøller, R., Kõljalg, U., Pennanen, T., Rosendahl, S., Stenlid, J., et al. (2013) · New Phytologist · doi:10.1111/nph.12243 · Cited by 892
Daniel, R. (2005) · Nature Reviews Microbiology · doi:10.1038/nrmicro1160 · Cited by 862
Quantification of denitrifying bacteria in soils by nirK gene targeted real-time PCR
Henry, S., Baudoin, E., López-Gutiérrez, J. C., Martin-Laurent, F., Brauman, A., & Philippot, L. (2004) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2004.07.002 · Cited by 698
Self-citationDo diet and taxonomy influence insect gut bacterial communities?
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Metadata
- Authors
- Martin-Laurent, F., Philippot, L., Hallet, S., Chaussod, R., Germon, J. C., Soulas, G., & Catroux, G.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2001-05
- Volume
- 67
- Issue
- 5
- Pages
- 2354-2359
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2119686278
- Cited by
- 727
- References
- 0
- FWCI
- 22.42
- Type
- journal article
- Language
- en
- Open access
- green
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- The paper
- Martin-Laurent, F., Philippot, L., Hallet, S., Chaussod, R., Germon, J. C., Soulas, G., & Catroux, G. (2001). DNA Extraction from Soils: Old Bias for New Microbial Diversity Analysis Methods. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.67.5.2354-2359.2001
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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