Cited by 251Microbial Metabolic Engineering and BioproductionBiofuel production and bioconversionFungal and yeast genetics research

The DOI matches its Crossref record; 37 of the 38 references it cites were found in a registry or an index.

Evolutionary Engineering of Saccharomyces cerevisiae for Anaerobic Growth on Xylose

Open access

Sonderegger, Marco, Sauer, Uwe·2003·Applied and Environmental Microbiology

DOI 10.1128/aem.69.4.1990-1998.2003PMC PMC154834

Open this paper in CoChat — free account

Abstract

Xylose utilization is of commercial interest for efficient conversion of abundant plant material to ethanol. Perhaps the most important ethanol-producing organism, Saccharomyces cerevisiae , however, is incapable of xylose utilization. While S. cerevisiae strains have been metabolically engineered to utilize xylose, none of the recombinant strains or any other naturally occurring yeast has been able to grow anaerobically on xylose. Starting with the recombinant S. cerevisiae strain TMB3001 that overexpresses the xylose utilization pathway from Pichia stipitis , in this study we developed a selection procedure for the evolution of strains that are capable of anaerobic growth on xylose alone. Selection was successful only when organisms were first selected for efficient aerobic growth on xylose alone and then slowly adapted to microaerobic conditions and finally anaerobic conditions, which indicated that multiple mutations were necessary. After a total of 460 generations or 266 days of selection, the culture reproduced stably under anaerobic conditions on xylose and consisted primarily of two subpopulations with distinct phenotypes. Clones in the larger subpopulation grew anaerobically on xylose and utilized both xylose and glucose simultaneously in batch culture, but they exhibited impaired growth on glucose. Surprisingly, clones in the smaller subpopulation were incapable of anaerobic growth on xylose. However, as a consequence of their improved xylose catabolism, these clones produced up to 19% more ethanol than the parental TMB3001 strain produced under process-like conditions from a mixture of glucose and xylose.

Integrity signals

Registry

Crossref · 10.1128/aem.69.4.1990-1998.2003

Cites

37 of 37 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Effect of benzoic acid on metabolic fluxes in yeasts: A continuous‐culture study on the regulation of respiration and alcoholic fermentation
Verduyn, C., Postma, E., Scheffers, W. A., & van Dijken, J. P. (1992) · Yeast · doi:10.1002/yea.320080703 · Cited by 1,510
Genetically Engineered Saccharomyces Yeast Capable of Effective Cofermentation of Glucose and Xylose
Ho, N. W. Y., Chen, Z., & Brainard, A. P. (1998) · Applied and Environmental Microbiology · doi:10.1128/aem.64.5.1852-1859.1998 · Cited by 541
Improvement of microbial strains and fermentation processes
Parekh, S. R., Vinci, V. A., & Strobel, R. J. (2000) · Applied Microbiology and Biotechnology · doi:10.1007/s002530000403 · Cited by 536
Xylose fermentation by Saccharomyces cerevisiae
K�tter, P., & Ciriacy, M. (1993) · Applied Microbiology and Biotechnology · doi:10.1007/bf00167144 · Cited by 507
Metabolic Engineering of Saccharomyces cerevisiae
Østergaard, S., Lisbeth Olsson, A., & Nielsen, J. (2000) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.64.1.34-50.2000 · Cited by 462
Metabolic engineering applications to renewable resource utilization
Aristidou, A. A., & Penttilä, M. E. (2000) · Current Opinion in Biotechnology · doi:10.1016/s0958-1669(00)00085-9 · Cited by 452
Directed evolution of biocatalysts
Arnold, F. H., & Volkov, A. A. (1999) · Current Opinion in Chemical Biology · doi:10.1016/s1367-5931(99)80010-6 · Cited by 411
Anaerobic Xylose Fermentation by Recombinant Saccharomyces cerevisiae Carrying XYL1 , XYL2 , and XKS1 in Mineral Medium Chemostat Cultures
Lantz, A. E., Christensson, C., Wahlbom, C. F., & Hahn‐Hägerdal, B. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.8.3381-3386.2000 · Cited by 392
Genome shuffling of Lactobacillus for improved acid tolerance
Patnaik, R., Louie, S., Gavrilovic, V., Perry, K., Stemmer, W. P. C., Ryan, C. M., & del Cardayré, S. B. (2002) · Nature Biotechnology · doi:10.1038/nbt0702-707 · Cited by 377
Microbial evolution in a simple unstructured environment: genetic differentiation in Escherichia coli.
Rosenzweig, R. F., Sharp, R. R., Treves, D. S., & Adams, J. (1994) · Genetics · doi:10.1093/genetics/137.4.903 · Cited by 357

Flagged first, then by citations

Show all 38 references

1 of the 38 references couldn’t be matched to a known paper.

Sorted by citations

Items 101–200 of 251

Biomass Conversion Inhibitors andIn SituDetoxification
Liu, Z. L., & Blaschek, H. P. (2010) · doi:10.1002/9780470750025.ch12 · Cited by 66
Construction of a xylose-fermenting Saccharomyces cerevisiae strain by combined approaches of genetic engineering, chemical mutagenesis and evolutionary adaptation
Liu, E., & Hu, Y. F. (2009) · Biochemical Engineering Journal · doi:10.1016/j.bej.2009.10.011 · Cited by 65
Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris
Baumann, K., Adelantado, N., Lang, C., Mattanovich, D., & Ferrer, P. (2011) · Microbial Cell Factories · doi:10.1186/1475-2859-10-93 · Cited by 65
“cerevisiae cultivations (see for example [30]).”
Reversible amyloids of pyruvate kinase couple cell metabolism and stress granule disassembly
Cereghetti, G., Wilson-Zbinden, C., Kissling, V. M., Diether, M., Arm, A., Yoo, H., Piazza, I., Saad, S., Picotti, P., Drummond, D. A., et al. (2021) · Nature Cell Biology · doi:10.1038/s41556-021-00760-4 · Cited by 64
Self-citation
Measurement of intracellular metabolites of primary metabolism and adenine nucleotides in chemostat cultivated Penicillium chrysogenum
Nasution, U., van Gulik, W. M., Kleijn, R. J., van Winden, W. A., Proell, A. M., & Heijnen, J. J. (2006) · Biotechnology and Bioengineering · doi:10.1002/bit.20842 · Cited by 64
d ‐Xylose consumption by nonrecombinant Saccharomyces cerevisiae : A review
Lagos, M. A. P., Ortiz, J. P. D., Velásquez, M., & Stambuk, B. U. (2019) · Yeast · doi:10.1002/yea.3429 · Cited by 65
Metabolic engineering of Saccharomyces cerevisiae to produce 1-hexadecanol from xylose
Guo, W., Sheng, J., Zhao, H., & Feng, X. (2016) · Microbial Cell Factories · doi:10.1186/s12934-016-0423-9 · Cited by 63
“cerevisiae successfully [33–35].”
Brazilian potential for biomass ethanol : Challenge of using hexose and pentose co-fermenting yeast strains
Stambuk, B. U., Eleuthério, E. C. A., Pardo, L. M. F., Souto-Maior, A. M., & Bon, E. P. S. (2008) · Journal of Scientific & Industrial Research · Cited by 62
Recent advances in the microbial production of squalene
Paramasivan, K., & Mutturi, S. (2022) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-022-03273-w · Cited by 60
“It entails a process of continuous evolution with a selective gain for the variant exhibiting the desired trait (Sonderegger and Sauer 2003; Çakar et al.”
Background
Establishment of l-arabinose fermentation in glucose/xylose co-fermenting recombinant Saccharomyces cerevisiae 424A(LNH-ST) by genetic engineering
Bera, A. K., Sedlák, M., Khan, A. A. P., & Ho, N. W. Y. (2010) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-010-2609-0 · Cited by 60
“Finally, the use of evolutionary engineering (Sonderegger and Sauer 2003; Kuyper et al. 2004; Wisselink et al. 2009) can also be applied for further improvement of our strain.”
Background
D ‐Xylose assimilation via the W eimberg pathway by solvent‐tolerant P seudomonas taiwanensis VLB 120
Köhler, K. A. K., Blank, L. M., Frick, O., & Schmid, A. (2014) · Environmental Microbiology · doi:10.1111/1462-2920.12537 · Cited by 59
Evolutionary adaptation of Kluyveromyces marxianus strain for efficient conversion of whey lactose to bioethanol
Saini, P., Beniwal, A., Kokkiligadda, A., & Vij, S. (2017) · Process Biochemistry · doi:10.1016/j.procbio.2017.07.013 · Cited by 59
Comparative Study of Natural and Artificial Flavoring Agents and Dyes
Dikshit, R., & Tallapragada, P. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-811518-3.00003-x · Cited by 59
Isolation of cobalt hyper-resistant mutants of Saccharomyces cerevisiae by in vivo evolutionary engineering approach
Çakar, Z. P., Alkım, C., Turanlı-Yıldız, B., Tokman, N., Akman, S., Sarıkaya, M., Tamerler, C., Benbadis, L., & François, J. (2009) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2009.06.024 · Cited by 57
Engineering Saccharomyces cerevisiae for next generation ethanol production
den Haan, R., Kroukamp, H., Mert, M. J., Viljoen‐Bloom, M., Görgens, J. F., & van Zyl, W. H. (2013) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.4068 · Cited by 57
Fermentation of glucose-xylose-arabinose mixtures by a synthetic consortium of single-sugar-fermenting Saccharomyces cerevisiae strains
Verhoeven, M. D., de Valk, S. C., Daran, J.-M. G., van Maris, A. J. A., & Pronk, J. T. (2018) · FEMS Yeast Research · doi:10.1093/femsyr/foy075 · Cited by 56
Transcription Analysis of Recombinant Saccharomyces cerevisiae Reveals Novel Responses to Xylose
Salusjärvi, L., Pitkänen, J., Aristidou, A. A., Ruohonen, L., & Penttilä, M. E. (2006) · Applied Biochemistry and Biotechnology · doi:10.1385/abab:128:3:237 · Cited by 56
Engineering of Saccharomyces cerevisiae for co-fermentation of glucose and xylose: Current state and perspectives
Qiu, Y., Wu, M., Bao, H., Liu, W., & Shen, Y. (2023) · Engineering Microbiology · doi:10.1016/j.engmic.2023.100084 · Cited by 57
Understanding low radiation background biology through controlled evolution experiments
Lampe, N., Breton, V., Sarramia, D., Sime‐Ngando, T., & Biron, D. G. (2017) · Evolutionary Applications · doi:10.1111/eva.12491 · Cited by 53
“…and protocols are already available, such as the prokaryote E. coli (Barrick et al., 2009; Lenski, Rose, Simpson, & Tadler, 1991), the eukaryote S. cerivasae (Ferea, Botstein, Brown, & Rosenzweig, 1999; Sonderegger & Sauer, 2003) and even the fruit fly Drosophila melanogaster (Burke et al., 2010).”
Method
Identification of common traits in improved xylose‐growing Saccharomyces cerevisiae for inverse metabolic engineering
Bengtsson, O., Jeppsson, M., Sonderegger, M., Parachin, N. S., Sauer, U., Hahn‐Hägerdal, B., & Gorwa‐Grauslund, M. F. (2008) · Yeast · doi:10.1002/yea.1638 · Cited by 50
“The laboratory strains C1 and C5 have been acquired by EMS mutagenesis and adaptation to anaerobic conditions on xylose in continuous culture (Sonderegger and Sauer, 2003) and BH42 has been obtained by breeding between industrial strain segregants and TMB3001 (Sonderegger et al.”
BackgroundInfluentialSelf-citation
Harnessing Genetic Diversity in Saccharomyces cerevisiae for Fermentation of Xylose in Hydrolysates of Alkaline Hydrogen Peroxide-Pretreated Biomass
Sato, T. K., Liu, T., Parreiras, L. S., Williams, D. L., Wohlbach, D. J., Bice, B. D., Ong, I. M., Breuer, R. J., Li, Q., Busalacchi, D., et al. (2013) · Applied and Environmental Microbiology · doi:10.1128/aem.01885-13 · Cited by 50
“NAD cofactor for xylitol dehydrogenase activity (17).”
Comparative transcriptomes reveal novel evolutionary strategies adopted by Saccharomyces cerevisiae with improved xylose utilization capability
Zeng, W.-Y., Tang, Y., Gou, M., Sun, Z.-Y., Xia, Z., & Kida, K. (2016) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-016-8046-y · Cited by 50
Lignocellulosic Biomass Conversion to Ethanol bySaccharomyces
Liu, Z. L., Badal Chandra, S. A. H. A., & Slininger, P. J. (2014) · ASM Press eBooks · doi:10.1128/9781555815547.ch2 · Cited by 49
Enhancement in xylose utilization using Kluyveromyces marxianus NIRE-K1 through evolutionary adaptation approach
Sharma, N. K., Behera, S., Arora, R., & Kumar, S. (2016) · Bioprocess and Biosystems Engineering · doi:10.1007/s00449-016-1563-3 · Cited by 48
“Sonderegger and Sauer [43] generated two evolutionary strains of recombinant S.”
Background
Metabolic engineering considerations for the heterologous expression of xylose-catabolic pathways in Saccharomyces cerevisiae
Jeong, D., Oh, E. J., Ko, J. K., Nam, J., Park, H.-S., Jin, Y., Lee, E. J., & Kim, S. R. (2020) · PLoS ONE · doi:10.1371/journal.pone.0236294 · Cited by 46
“Adaptive evolution have been the most commonly used and the most effective approach to improve both the strains expressing the oxidoreductase pathway [3,9,10] and the strains expressing the isomerase pathway [11–13].”
Method
Inverse metabolic engineering with phosphagen kinase systems improves the cellular energy state
Sauer, U., & Schlattner, U. (2004) · Metabolic Engineering · doi:10.1016/j.ymben.2003.11.004 · Cited by 45
“Recent examples of such evolved phenotypes with applied interest include improved pentose metabolism (Becker and Boles, 2003; Sonderegger and Sauer, 2003), incorporation of unnatural amino acids (Bacher and Ellington, 2001), and pH (Patnaik et al.”
BackgroundSelf-citation
Genome engineering for breaking barriers in lignocellulosic bioethanol production
Ulaganathan, K., Goud, S., Reddy, M., & Ulaganathan, K. (2017) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2017.01.028 · Cited by 45
The expression in Saccharomyces cerevisiae of a glucose/xylose symporter from Candida intermedia is affected by the presence of a glucose/xylose facilitator
Leandro, M. J., Spencer‐Martins, I., & Gonçalves, P. M. (2008) · Microbiology · doi:10.1099/mic.0.2007/015511-0 · Cited by 45
“The ura2 mutants of S. cerevisiae strains TMB 3001 and C5 (Sonderegger & Sauer, 2003) were transformed with the high-copynumber plasmid pHXT7-GXS1 (Leandro et al., 2006), producing strains MJY7 and MJY9, respectively.”
BackgroundMethodInfluential
Evolutionary engineering of a wine yeast strain revealed a key role of inositol and mannoprotein metabolism during low-temperature fermentation
López‐Malo, M., García‐Ríos, E., Melgar, B., Sánchez, M., Dunham, M. J., & Guillamón, J. M. (2015) · BMC Genomics · doi:10.1186/s12864-015-1755-2 · Cited by 45
“Evolutionary engineering has been used for generating new industrial strains [13–15].”
Method
Single Amino Acid Substitutions in HXT2.4 from Scheffersomyces stipitis Lead to Improved Cellobiose Fermentation by Engineered Saccharomyces cerevisiae
Ha, S.-J., Kim, H., Lin, Y., Jang, M.-U., Galazka, J. M., Kim, T., Cate, J. H. D., & Jin, Y. (2012) · Applied and Environmental Microbiology · doi:10.1128/aem.03253-12 · Cited by 40
Process intensification through microbial strain evolution: mixed glucose-xylose fermentation in wheat straw hydrolyzates by three generations of recombinant Saccharomyces cerevisiae
Novy, V., Krahulec, S., Wegleiter, M., Müller, G., Longus, K., Klimacek, M., & Nidetzky, B. (2014) · Biotechnology for Biofuels · doi:10.1186/1754-6834-7-49 · Cited by 39
“Consequences of evolutionary engineering were assessed in pure sugar substrate fermentations [23,26,39], but also in lignocellulose hydrolyzate conversions [24,34].”
BackgroundMethodInfluential
Metabolic engineering of a xylose pathway for biotechnological production of glycolate in Escherichia coli
Liu, M., Ding, Y., Xian, M., & Zhao, G. (2018) · Microbial Cell Factories · doi:10.1186/s12934-018-0900-4 · Cited by 39
“Bacteria like Escherichia coli generally employ a one-step pathway using xylose isomerase (XI) enzyme, whereas yeasts and mycelial fungi need two enzymatic–catalytic steps by reduction reaction of d-xylose reductase (XR) and oxidation reaction of xylitol dehydrogenase (XDH) [18, 19].”
Background
Inverse metabolic engineering of Bacillus subtilis for xylose utilization based on adaptive evolution and whole-genome sequencing
Zhang, B., Li, N., Wang, Z., Ya-jie Tang, A., Chen, T., & Zhao, X. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-6131-7 · Cited by 35
Ethanol Production from Traditional and Emerging Raw Materials
Rudolf, A., Karhumaa, K., & Hahn‐Hägerdal, B. (2009) · Springer eBooks · doi:10.1007/978-1-4020-8292-4_23 · Cited by 34
Implementation of a transhydrogenase-like shunt to counter redox imbalance during xylose fermentation in Saccharomyces cerevisiae
Suga, H., Matsuda, F., Hasunuma, T., Ishii, J., & Kondo, A. (2012) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-012-4298-3 · Cited by 32
“…Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan F. Matsuda :A. Kondo RIKEN Biomass Engineering Program, 1-7-22 Suehirocho, Tsurumi, Yokohama 230-0045, Japan Katahira et al. 2008; Sonderegger and Sauer 2003; Verho et al. 2003; Watanabe et al. 2007).”
Background
Cell Aggregation and Aerobic Respiration Are Important for Zymomonas mobilis ZM4 Survival in an Aerobic Minimal Medium
Jones-Burrage, S. E., Kremer, T. A., & McKinlay, J. B. (2019) · Applied and Environmental Microbiology · doi:10.1128/aem.00193-19 · Cited by 33
“tested the addition of a readily available vitamin mixture (40) with B vitamins within the”
Method
The role that lignocellulosic feedstocks and various biorefining technologies can play in meeting Ireland's biofuel targets
Hayes, D. J. M., & Hayes, M. H. B. (2009) · Biofuels Bioproducts and Biorefining · doi:10.1002/bbb.171 · Cited by 33
“5 Ethanol from hemicellulose sugars (% of theoretical) c, d 59 [41] 86 [35] 59 [41] 59 93.”
Method
Crabtree-negative characteristics of recombinant xylose-utilizing Saccharomyces cerevisiae
Souto-Maior, A. M., Runquist, D., & Hahn‐Hägerdal, B. (2009) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2009.06.022 · Cited by 33
APJ1 and GRE3 Homologs Work in Concert to Allow Growth in Xylose in a Natural Saccharomyces sensu stricto Hybrid Yeast
Schwartz, K., Wenger, J. W., Dunn, B. L., & Sherlock, G. J. (2012) · Genetics · doi:10.1534/genetics.112.140053 · Cited by 31
“…has been made over the past 30 years to address this issue, and through the use of metabolic engineering and directed evolution (Ho et al. 1998; Sonderegger and Sauer 2003; Kuyper et al. 2004; Matsushika et al. 2009; Kim et al. 2010; Ha et al. 2011) strains of S. cerevisiae that have the…”
Background
Strain improvement of Candida tropicalis for the production of xylitol: biochemical and physiological characterization of wild-type and mutant strain CT-OMV5.
Rao, R. S., Jyothi, C. P., Prakasham, R. S., Rao, C. S., Sarma, P. N., & Rao, L. V. (2006) · PubMed · Cited by 31
Second Generation Bioethanol Production: The State of Art
Hemansi, Gupta, R., Yadav, G., Kumar, G., Yadav, A., Saini, J. K., & Kuhad, R. C. (2018) · Biofuel and biorefinery technologies · doi:10.1007/978-3-319-94797-6_8 · Cited by 31
Selection of quiescent Escherichia coli with high metabolic activity
Sonderegger, M., Schümperli, M., & Sauer, U. (2005) · Metabolic Engineering · doi:10.1016/j.ymben.2004.05.005 · Cited by 31
Self-citation
Proteome analysis of the xylose‐fermenting mutant yeast strain TMB 3400
Karhumaa, K., Påhlman, A., Hahn‐Hägerdal, B., Levander, F., & Gorwa‐Grauslund, M. F. (2009) · Yeast · doi:10.1002/yea.1673 · Cited by 30
“…methods such as mutagenesis, adaptation and evolutionary engineering have also been used to generate improved yeast strains (Attfield and Bell, 2006; Becker and Boles, 2003; Karhumaa et al., 2005; Kuyper et al., 2005; Pitkänen et al., 2005; Sonderegger and Sauer, 2003; Wahlbom et al., 2003b).”
MethodInfluential
Engineering stress tolerance of Escherichia coli by stress‐induced mutagenesis (SIM)‐based adaptive evolution
Zhu, L., Cai, Z., Zhang, Y., & Li, Y. (2013) · Biotechnology Journal · doi:10.1002/biot.201300277 · Cited by 30
Tracking strategic developments for conferring xylose utilization/fermentation by Saccharomyces cerevisiae
Sharma, S., & Arora, A. (2020) · Annals of Microbiology · doi:10.1186/s13213-020-01590-9 · Cited by 30
“This strategy includes several cycles of random mutations and sequential selection, which create genetic mutations involving the desired ones (Sauer and Schlattner 2004) and can be very effective in strain improvement (Overkamp et al. 2002; Sonderegger and Sauer 2003; Shi et al. 2014).”
Background
Adaptive evolution of engineered yeast for squalene production improvement and its genome‐wide analysis
Paramasivan, K., Abdulla, A., Gupta, N., & Mutturi, S. (2021) · Yeast · doi:10.1002/yea.3559 · Cited by 27
“Evolutionary engineering involves the process of prolonged growth with a selective advantage towards the strain that expresses the desirable trait (Çakar et al., 2005; Sonderegger & Sauer, 2003).”
Background
Recombinant Diploid Saccharomyces cerevisiae Strain Development for Rapid Glucose and Xylose Co-Fermentation
Liu, T., Huang, S., & Geng, A. (2018) · Fermentation · doi:10.3390/fermentation4030059 · Cited by 27
Re-routing the hemicellulosic fraction of lignocellulosic biomass toward value added products: A pragmatic bio refinery approach
Chandukishore, T., Das, S., Das, P. K., Veeranki, V. D., & Prabhu, A. A. (2024) · Journal of environmental chemical engineering · doi:10.1016/j.jece.2024.111971 · Cited by 25
Extracellular Phytase Production by the Wine Yeast S. cerevisiae (Finarome Strain) during Submerged Fermentation
Kłosowski, G., Mikulski, D., & Jankowiak, O. (2018) · Molecules · doi:10.3390/molecules23040848 · Cited by 25
Unreliable medical information on birth certificates.
Gore, D., Chez, R. A., Remmel, R. J., Harahan, M., Mock, M., & Yelverton, R. W. (2002) · PubMed · Cited by 25
Systematic Applications of Metabolomics in Metabolic Engineering
Dromms, R., & Styczynski, M. P. (2012) · Metabolites · doi:10.3390/metabo2041090 · Cited by 24
Engineering of the redox imbalance of Fusarium oxysporum enables anaerobic growth on xylose
Panagiotou, G., Christakopoulos, P., Grotkjær, T., & Lisbeth Olsson, A. (2006) · Metabolic Engineering · doi:10.1016/j.ymben.2006.04.004 · Cited by 24
Evolution-based strategy to generate non-genetically modified organisms Saccharomyces cerevisiae strains impaired in sulfate assimilation pathway
Vero, L. D., Solieri, L., & Giudici, P. (2011) · Letters in Applied Microbiology · doi:10.1111/j.1472-765x.2011.03140.x · Cited by 23
“Alternatively, evolutionary approaches are valuable for the selection of new strains with specific phenotypes, such as resistance to various stresses or efficient substrate utilization (Sonderegger and Sauer 2003; Kuyper et al. 2004; Cakar et al. 2009; Cadière et al. 2011).”
Background
Synthetic biology-driven microbial production of folates: Advances and perspectives
Yang, H. M., Zhang, X., Liu, Y., Liu, L., Li, J., Du, G., & Chen, J. (2020) · Bioresource Technology · doi:10.1016/j.biortech.2020.124624 · Cited by 21
Stepwise metabolic adaption from pure metabolization to balanced anaerobic growth on xylose explored for recombinant Saccharomyces cerevisiae
Klimacek, M., Kirl, E., Krahulec, S., Longus, K., Novy, V., & Nidetzky, B. (2014) · Microbial Cell Factories · doi:10.1186/1475-2859-13-37 · Cited by 21
“Following strains were used to construct the graph (starting with the lowest μ): BP10001 (this study), TMB3001-C1 [8], TMB3415 [14], IBB10A02 (this study), RWB202-AFX [9], TMB3421 [15], IBB10B05 (this study), TMB3422 [15], H131-A3SB-3 [17], TMB3420 [15], RWB17 [13], H131-A3CS [17], RWB18 [16], H131-A3-ALCS [17].”
BackgroundMethod
Non-Genetic Engineering Approaches for Isolating and Generating Novel Yeasts for Industrial Applications
Chambers, P. J., Bellon, J. R., Schmidt, S. A., Varela, C., & Pretorius, I. S. (2009) · Springer eBooks · doi:10.1007/978-1-4020-8292-4_20 · Cited by 21
High-Efficiency Biocatalytic Conversion of Thebaine to Codeine
Li, X., Krysiak-Baltyn, K., Richards, L., Jarrold, A., Stevens, G. W., Bowser, T. A., Speight, R. E., & Gras, S. L. (2020) · ACS Omega · doi:10.1021/acsomega.0c00282 · Cited by 19
Genomics and transcriptomics analysis reveals the mechanism of isobutanol tolerance of a laboratory evolved Lactococcus lactis strain
Gupta, J. A., Thapa, S., Verma, M., Som, R., & Mukherjee, K. J. (2020) · Scientific Reports · doi:10.1038/s41598-020-67635-w · Cited by 19
In vivo evolutionary engineering of a boron-resistant bacterium: Bacillus boroniphilus
Şen, M., Yılmaz, Ü., Baysal, A., Akman, S., & Çakar, Z. P. (2011) · Antonie van Leeuwenhoek · doi:10.1007/s10482-011-9557-2 · Cited by 18
Evolutionary engineering of Phaffia rhodozyma for astaxanthin-overproducing strain
Gong, J., Duan, N., & Zhao, X. (2012) · Frontiers of Chemical Science and Engineering · doi:10.1007/s11705-012-1276-3 · Cited by 18
“4 Astaxanthin of evolved strain by cultured in medium with (1) ionone, (2) diphenylamine, (3) NaCl, (4) TiO2, (5) H2O2, (6) NaClO, and (7) the wild strain.”
Background
High Gravity Ethanol Fermentations and Yeast Tolerance
Bai, F., & Zhao, X. (2011) · Microbiology monographs · doi:10.1007/978-3-642-21467-7_5 · Cited by 18
Saccharomyces cerevisiae strain comparison in glucose–xylose fermentations on defined substrates and in high-gravity SSCF: convergence in strain performance despite differences in genetic and evolutionary engineering history
Novy, V., Wang, R., Westman, J. O., Franzén, C. J., & Nidetzky, B. (2017) · Biotechnology for Biofuels · doi:10.1186/s13068-017-0887-9 · Cited by 18
“Strain selection has mainly been based on aerobic [21, 22] or anaerobic growth on xylose [20, 23].”
Background
Better Yeast for Better Wine — Genetic Improvement of Saccharomyces Cerevisiae Wine Strains
Schuller, D. E. (2010) · Springer eBooks · doi:10.1007/978-90-481-3713-8_1 · Cited by 16
A constitutive catabolite repression mutant of a recombinantSaccharomyces cerevisiae strain improves xylose consumption during fermentation
Gururajan, V. T., Gorwa‐Grauslund, M. F., Hahn‐Hägerdal, B., Pretorius, I. S., & Otero, R. R. C. (2007) · Annals of Microbiology · doi:10.1007/bf03175055 · Cited by 16
“…Jin et al., 2002; Johansson and HahnHägerdal, 2002; Jeppsson et al., 2003; Verho et al., 2003) have been combined with a mutational approach (Wahlbom et al., 2003a) as well as evolutionary engineering (Sonderegger and Sauer, 2003), resulting in mutants that are able to grow on and ferment xylose.”
Background
Adaptive Evolution of Industrial Brewer’s Yeast Strains towards a Snowflake Phenotype
Kayacan, Y., Mieghem, T. V., Delvaux, F., Delvaux, F. R., & Willaert, R. (2020) · Fermentation · doi:10.3390/fermentation6010020 · Cited by 15
“cerevisiae [16], increasing tolerance of S.”
The deletion of YLR042c improves ethanolic xylose fermentation by recombinant Saccharomyces cerevisiae
Parachin, N. S., Bengtsson, O., Hahn‐Hägerdal, B., & Gorwa‐Grauslund, M. F. (2010) · Yeast · doi:10.1002/yea.1777 · Cited by 15
High-titer-ethanol production from cellulosic hydrolysate by an engineered strain of Saccharomyces cerevisiae during an in situ removal process reducing the inhibition of ethanol on xylose metabolism
Zhang, B., Sun, H., Li, J., Wan, Y., Li, Y., & Zhang, Y. (2016) · Process Biochemistry · doi:10.1016/j.procbio.2016.04.019 · Cited by 15
Genetic variation and expression changes associated with molybdate resistance from a glutathione producing wine strain of Saccharomyces cerevisiae
Mezzetti, F., Fay, J. C., Giudici, P., & Vero, L. D. (2017) · PLoS ONE · doi:10.1371/journal.pone.0180814 · Cited by 14
Engineering xylose fermentation in an industrial yeast: continuous cultivation as a tool for selecting improved strains
Basso, T. P., Procópio, D. P., Petrin, T. H. C., Giacon, T. G., Jin, Y., Basso, T. O., & Basso, L. C. (2023) · Letters in Applied Microbiology · doi:10.1093/lambio/ovad077 · Cited by 13
A high-throughput metabolomics method to predict high concentration cytotoxicity of drugs from low concentration profiles
Heux, S., Fuchs, T. J., Buhmann, J. M., Zamboni, N., & Sauer, U. (2011) · Metabolomics · doi:10.1007/s11306-011-0386-0 · Cited by 13
Self-citation
Evolutionary engineering of yeast for closed-circulating ethanol fermentation in PDMS membrane bioreactor
Ding, W., Xiao, Z., Tang, X., Deng, K., Fu, S., Jiang, Y., & Yuan, L. (2011) · Biochemical Engineering Journal · doi:10.1016/j.bej.2011.09.019 · Cited by 13
Evolutionary metabolic engineering
Koffas, M. A. G., & del Cardayre, S. (2005) · Metabolic Engineering · doi:10.1016/j.ymben.2005.01.001 · Cited by 13
Isolation and characterization of a mutant recombinant Saccharomyces cerevisiae strain with high efficiency xylose utilization
Tomitaka, M., Taguchi, H., Fukuda, K., Akamatsu, T., & Kida, K. (2013) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2013.05.027 · Cited by 12
Enhanced xylose fermentation capacity related to an altered glucose sensing and repression network in a recombinantSaccharomyces cerevisiae
Shen, Y., Hou, J., & Bao, X. (2013) · Bioengineered · doi:10.4161/bioe.25542 · Cited by 12
A High‐Throughput Workflow for CRISPR/Cas9 Mediated Combinatorial Promoter Replacements and Phenotype Characterization in Yeast
Kuivanen, J., Holmström, S., Lehtinen, B., Penttilä, M. E., & Jäntti, J. (2018) · Biotechnology Journal · doi:10.1002/biot.201700593 · Cited by 12
Incorporating comparative genomics into the design–test–learn cycle of microbial strain engineering
Sardi, M. I., & Gasch, A. P. (2017) · FEMS Yeast Research · doi:10.1093/femsyr/fox042 · Cited by 15
Metabolic and evolutionary engineering research in Turkey and beyond
Çakar, Z. P. (2009) · Biotechnology Journal · doi:10.1002/biot.200800332 · Cited by 10
“Thus, growth on xylose under anaerobic conditions was achieved at the end of the evolutionary engineering application [124].”
Background
Fuel Ethanol Production from Lignocellulosic Raw Materials Using Recombinant Yeasts
Stanley, G. A., & Hahn‐Hägerdal, B. (2010) · doi:10.1002/9780470750025.ch13 · Cited by 10
High consistency enzymatic hydrolysis of lignocellulose
Qin, W. (2010) · cIRcle (University of British Columbia) · doi:10.14288/1.0069976 · Cited by 10
“Therefore, metabolic engineering of xylose fermentation in Saccharomyces cerevisiae is an attractive approach (Sonderegger and Sauer 2003).”
Background
Genetic Engineering for Strain Improvement in Filamentous Fungi
Garrigues, S., Martínez-Reyes, N., & de Vries, R. P. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-819990-9.00006-8 · Cited by 10
Biotechnological Advances in Lignocellulosic Ethanol Production
Bhatia, L., Chandel, A. K., Singh, A. K., & Singh, O. V. (2018) · Springer eBooks · doi:10.1007/978-3-319-95480-6_3 · Cited by 8
Using microorganisms to brew biofuels
Rao, R. P., Dufour, N. P., & Swana, J. (2011) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/s11627-011-9374-3 · Cited by 8
Isolation and characterization of xylitol-assimilating mutants of recombinant Saccharomyces cerevisiae
Tani, T., Taguchi, H., Fujimori, K. E., Sahara, T., Ohgiya, S., Kamagata, Y., & Akamatsu, T. (2016) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2016.03.008 · Cited by 8
Integration of Ethanol Fermentation with Second Generation Biofuels Technologies
Häggström, C., Rova, U., Brandberg, T., & Hodge, D. B. (2014) · Elsevier eBooks · doi:10.1016/b978-0-444-59498-3.00008-7 · Cited by 8
Strain Development by Whole-Cell Directed Evolution
Si, T., Lian, J., & Zhao, H. (2017) · Springer eBooks · doi:10.1007/978-3-319-50413-1_7 · Cited by 8
Evaluation of phytic acid utilization by S. cerevisiae strains used in fermentation processes and biomass production
Mikulski, D., & Kłosowski, G. (2016) · Journal of Basic Microbiology · doi:10.1002/jobm.201600311 · Cited by 7
“cerevisiae calibration curve using Pharo 300 spectrophotometer by Merck 1 [19].”
Method
A Microbial Perspective on Ethanolic Lignocellulose Fermentation
Parachin, N. S., Hahn‐Hägerdal, B., & Bettiga, M. (2011) · Elsevier eBooks · doi:10.1016/b978-0-08-088504-9.00390-1 · Cited by 7
Adaptive Evolution of Saccharomyces cerevisiae in a Continuous and Closed Circulating Fermentation (CCCF) System Coupled with PDMS Membrane Pervaporation
Chen, C., Tang, X., Xiao, Z., Zhou, Y., Jiang, Y., & Fu, S. (2013) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-013-0142-1 · Cited by 7
Development and characterisation of a recombinantSaccharomyces cerevisiae mutant strain with enhanced xylose fermentation properties
Gururajan, V. T., Rensburg, P. V., Hahn‐Hägerdal, B., Pretorius, I. S., & Otero, R. R. C. (2007) · Annals of Microbiology · doi:10.1007/bf03175361 · Cited by 6
“…to using targeted metabolic engineering approaches (Bailey, 1991) to generate xylose-fermenting strains of S. cerevisiae, strains overexpressing XR, XDH and XK have been further improved by random evolutionary engineering strategies (Sauer, 2001; Sonderegger and Sauer, 2003; Wahlbom et al., 2003a).”
Background
Metabolomics Analysis Reveals Global Metabolic Changes in the Evolved E. coli Strain with Improved Growth and 1-Butanol Production in Minimal Medium
Laviña, W. A., Sakurai, S. S. M., Pontrelli, S., Putri, S. P., & Fukusaki, E. (2020) · Metabolites · doi:10.3390/metabo10050192 · Cited by 6
Improving Yeast Strains for Pentose Hexose Co-fermentation: Successes and Hurdles
Sharma, S., Sharma, S., Singh, S., Lata, L., & Arora, A. (2016) · Springer proceedings in energy · doi:10.1007/978-81-322-2773-1_3 · Cited by 6
Transcriptome and proteome analysis of xylose-metabolising Saccharomyces cerevisiae
Salusjärvi, L. (2008) · Työväentutkimus Vuosikirja · Cited by 6
Biotechnological approaches concerning valorisation of fungal enzymes in agro-industrial waste management
Atta, O. M., Chen, L., Mohamed, H., Ullah, S., Abo-Kadoum, M. A., Abdel-Aziz, A. E., Taleb, K., Mosa, M. A., & Hassane, A. M. A. (2025) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2025.108585 · Cited by 5
Evolutionary Engineering for Industrial Microbiology
Vanee, N., Fisher, A. B., & Fong, S. S. (2012) · Sub-cellular biochemistry/Subcellular biochemistry · doi:10.1007/978-94-007-5055-5_3 · Cited by 5
Artificial transcription regulator as a tool for improvement of cellular property in Saccharomyces cerevisiae
Lee, S., Kim, E., Kim, J., & Oh, M. (2012) · Chemical Engineering Science · doi:10.1016/j.ces.2012.09.007 · Cited by 5
A dynamic and steady state metabolome study of central metabolism and its relation with the penicillin biosynthesis pathway in Penicillium chrysogenum
Nasution, U. (2007) · Research Repository (Delft University of Technology) · Cited by 5
Efficiency and Emissions Study of a Residential Micro–cogeneration System Based on a Stirling Engine and Fuelled by Diesel and Ethanol
Farra, N. (2010) · TSpace (University of Toronto) · Cited by 5
Unbiased metabolic flux inference through combined thermodynamic and 13 C flux analysisPreprint
Saldida, J., Muntoni, A. P., Martino, D. D., Hubmann, G., Niebel, B., Schmidt, A. M., Braunstein, A., Milias‐Argeitis, A., & Heinemann, M. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.06.29.177063 · Cited by 6
“The normalised metabolite concentrations were converted into intracellular concentration using the dry weight / OD ratio of 0.52 (Sonderegger & Sauer, 2003) and the cell volume / dry weight ratio of 2 mL gCDW-1 (Van Urk et al, 1988).”
MethodInfluential
Genomic adaptation of Saccharomyces cerevisiae to inhibitors for lignocellulosic biomass conversion to ethanol.
Liu, Z. L., & Song, M. Z. (2009) · CABI eBooks · doi:10.1079/9781845935344.0136 · Cited by 4
Yeast Expression Systems: Current Status and Future Prospects
Adivitiya, Dagar, V. K., & Khasa, Y. P. (2017) · Springer eBooks · doi:10.1007/978-981-10-2621-8_9 · Cited by 4

Authors: Sonderegger, M., & Sauer, U.
Affiliations: ETH Zurich (CH)
Corresponding author: Sauer, Uwe
ORCID: Sauer, Uwe
Venue: Applied and Environmental Microbiology
Publisher: American Society for Microbiology
Published: 2003-04
Volume: 69
Issue: 4
Pages: 1990-1998
ISSN: 0099-2240 (ISSN-L)
1098-5336
DOI: 10.1128/aem.69.4.1990-1998.2003
PMC: PMC154834
OpenAlex: W2115470699
Cited by: 251
References: 38
Citations by year: 2026: 2 · 2025: 6 · 2024: 2 · 2023: 5 · 2022: 3 · 2021: 4 · 2020: 14 · 2019: 6 · 2018: 8 · 2017: 17 · 2016: 13 · 2015: 5 · 2014: 12 · 2013: 14 · 2012: 18
FWCI: 3.68
Type: journal article
Language: en
Open access: green
europepmc.org
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Applied and Environmental Microbiology (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: European Commission
Office Fédéral de l'Education et de la Science
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords: Saccharomyces cerevisiae, xylose utilization, anaerobic growth, evolutionary engineering, ethanol production, glucose utilization, xylose catabolism, recombinant yeast strains
MeSH terms: Aldehyde Reductase, Anaerobiosis, Culture Media, Genetic Engineering, Pichia, Recombination, Genetic, Saccharomyces cerevisiae, Sugar Alcohol Dehydrogenases, Xylose, Phosphotransferases (Alcohol Group Acceptor), Evolution, Molecular, D-Xylulose Reductase

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Evolutionary Engineering of Saccharomyces cerevisiae for Anaerobic Growth on Xylose

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks