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External pH and Nitrogen Source Affect Secretion of Pectate Lyase by Colletotrichum gloeosporioides
Open accessAbstract
Accumulation of ammonia and associated tissue alkalinization predispose fruit to attack by Colletotrichumgloeosporioides. As the external pH increases from 4.0 to 6.0, pectate lyase (PL) and other extracellular proteins are secreted and accumulate. At pH 4.0 neither pelB (encoding PL) transcription nor PL secretion were detected; however, they were detected as the pH increased. Nitrogen assimilation also was required for PL secretion at pH 6.0. Both inorganic and organic nitrogen sources enhanced PL secretion at pH 6.0, but neither was sufficient for PL secretion at pH 4.0. Sequence analysis of the 5′ upstream region of the pelB promoter revealed nine putative consensus binding sites for the Aspergillus transcription factor PacC. Consistent with this result, the transcript levels of pac1 (the C. gloeosporioides pacC homologue) and pelB increased in parallel as a function of pH. Our results suggest that the ambient pH and the nitrogen source are independent regulatory factors for processes linked to PL secretion and virulence of C. gloeosporioides .
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The expression of pectate lyase by pelB induces the degradation of the plant cell wall in a pH-dependent manner under the regulation of pacC.
“The expression of pectate lyase by pelB induces the degradation of the plant cell wall in a pH-dependent manner under the regulation of pacC (Drori et al., 2003; Kramer-Haimovich et al., 2006).”
From Mango anthracnose disease: the current situation and direction for future research (2023) · cites this paper as Drori et al., 2003
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Mango anthracnose disease: the current situation and direction for future research
Dofuor, A. K., Quartey, N. K.-A., Osabutey, A. F., Antwi-Agyakwa, A. K., Asante, K., Boateng, B. O., Ablormeti, F. K., Lutuf, H., Osei-Owusu, J., Osei, J. H. N., et al. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2023.1168203 · Cited by 103
“The expression of pectate lyase by pelB induces the degradation of the plant cell wall in a pH-dependent manner under the regulation of pacC (Drori et al., 2003; Kramer-Haimovich et al., 2006).”
Background
Metadata
- Authors
- Drori, N., Kramer-Haimovich, H., Rollins, J., Dinoor, A., Okon, Y., Pines, O., & Prusky, D.
- Affiliations
- Hebrew University of Jerusalem (IL)
- University of Florida (US)
- Agricultural Research Organization (IL)
- Corresponding author
- Prusky, D.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2003-06
- Volume
- 69
- Issue
- 6
- Pages
- 3258-3262
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC161482
- OpenAlex
- W2101516193
- Cited by
- 83
- References
- 0
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 2 · 2023: 5 · 2022: 2 · 2021: 2 · 2020: 6 · 2019: 3 · 2018: 5 · 2017: 2 · 2016: 4 · 2015: 1 · 2014: 2 · 2013: 3 · 2012: 6
- FWCI
- 0.81
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Pathogens and Fungal Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Colletotrichum gloeosporioides, pectate lyase, external pH, nitrogen source, nitrogen assimilation, extracellular protein secretion, PacC transcription factor, PAC1, promoter binding sites, fruit rot, plant pathogenic fungi, virulence
- MeSH terms
- Fungal Proteins, Hydrogen-Ion Concentration, Molecular Sequence Data, Nitrogen, Plant Diseases, Polysaccharide-Lyases, Transcription Factors, Transcription, Genetic, Gene Expression Regulation, Fungal, Sequence Analysis, DNA, Colletotrichum
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