The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Biocatalytic Production of Perillyl Alcohol from Limonene by Using a Novel Mycobacterium sp. Cytochrome P450 Alkane Hydroxylase Expressed in Pseudomonas putida
Open accessAbstract
A number of oxygenated monoterpenes present at low concentrations in plant oils have anticarcinogenic properties. One of the most promising compounds in this respect is (−)-perillyl alcohol. Since this natural product is present only at low levels in a few plant oils, an alternative, synthetic source is desirable. Screening of 1,800 bacterial strains showed that many alkane degraders were able to specifically hydroxylate l -limonene in the 7 position to produce enantiopure (−)-perillyl alcohol. The oxygenase responsible for this was purified from the best-performing wild-type strain, Mycobacterium sp. strain HXN-1500. By using N-terminal sequence information, a 6.2-kb ApaI fragment was cloned, which encoded a cytochrome P450, a ferredoxin, and a ferredoxin reductase. The three genes were successfully coexpressed in Pseudomonas putida by using the broad-host-range vector pCom8, and the recombinant converted limonene to perillyl alcohol with a specific activity of 3 U/g (dry weight) of cells. The construct was subsequently used in a 2-liter bioreactor to produce perillyl alcohol on a scale of several grams.
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Citations
Cites
The Carbon Monoxide-binding Pigment of Liver Microsomes
Omura, T., & Sato, R. (1964) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)82244-3 · Cited by 11,781
Cited by
Cytochrome P450 systems—biological variations of electron transport chains
Hannemann, F., Bichet, A., Ewen, K. M., & Bernhardt, R. (2007) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2006.07.017 · Cited by 759
A Global Survey of Mycobacterial Diversity in Soil
Walsh, C. M., Gebert, M. J., Delgado-Baquerizo, M., Maestre, F. T., & Fierer, N. (2019) · Applied and Environmental Microbiology · doi:10.1128/aem.01180-19 · Cited by 111
Odhiambo, C. A., Malico, A. A., & Williams, G. J. (2026) · RSC Advances · doi:10.1039/d6ra03129c · Cited by 1
Metadata
- Authors
- van Beilen, J. B., Holtackers, R., Lüscher, D., Bauer, U., Witholt, B., & Duetz, W. A.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2005-04
- Volume
- 71
- Issue
- 4
- Pages
- 1737-1744
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2158120631
- Cited by
- 172
- References
- 1
- FWCI
- 8.57
- Type
- journal article
- Language
- en
- Open access
- green
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- The paper
- van Beilen, J. B., Holtackers, R., Lüscher, D., Bauer, U., Witholt, B., & Duetz, W. A. (2005). Biocatalytic Production of Perillyl Alcohol from Limonene by Using a Novel Mycobacterium sp. Cytochrome P450 Alkane Hydroxylase Expressed in Pseudomonas putida. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.71.4.1737-1744.2005
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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