Fate of 14 C-Labeled Microbial Products Derived from Nitrifying Bacteria in Autotrophic Nitrifying Biofilms
Abstract
The cross-feeding of microbial products derived from 14 C-labeled nitrifying bacteria to heterotrophic bacteria coexisting in an autotrophic nitrifying biofilm was quantitatively analyzed by using microautoradiography combined with fluorescence in situ hybridization (MAR-FISH). After only nitrifying bacteria were labeled with [ 14 C]bicarbonate, biofilm samples were incubated with and without NH 4 + as a sole energy source for 10 days. The transfer of 14 C originally incorporated into nitrifying bacterial cells to heterotrophic bacteria was monitored with time by using MAR-FISH. The MAR-FISH analysis revealed that most phylogenetic groups of heterotrophic bacteria except the β- Proteobacteria showed significant uptake of 14 C-labeled microbial products. In particular, the members of the Chloroflexi were strongly MAR positive in the culture without NH 4 + addition, in which nitrifying bacteria tended to decay. This indicated that the members of the Chloroflexi preferentially utilized microbial products derived from mainly biomass decay. On the other hand, the members of the Cytophaga - Flavobacterium cluster gradually utilized 14 C-labeled products in the culture with NH 4 + addition in which nitrifying bacteria grew. This result suggested that these bacteria preferentially utilized substrate utilization-associated products of nitrifying bacteria and/or secondary metabolites of 14 C-labeled structural cell components. Our results clearly demonstrated that the coexisting heterotrophic bacteria efficiently degraded and utilized dead biomass and metabolites of nitrifying bacteria, which consequently prevented accumulation of organic waste products in the biofilm.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In a microbial community founded on an inorganic electron donor and autotrophy, organic carbon and nutrient-available forms of nitrogen, sulfur, and selenium become available through endogenous decay of the autotrophic biomass.
“An important aspect of a microbial community that is founded on an inorganic electron donor and autotrophy is that organic carbon (C) and nutrient-available forms of nitrogen (N), sulfur (S), and selenium (Se) become available through endogenous decay of the autotrophic biomass (Okabe et al. 2005, Zevin et al. 2016, Ontiveros-Valencia et al. 2018).”
From Microbial ecology of nitrate-, selenate-, selenite-, and sulfate-reducing bacteria in a H2-driven bioprocess (2024) · cites this paper as Okabe et al. 2005
The Discussion reports that nitrifying bacteria decayed without an added energy source, producing biomass-associated products; it also describes cell lysis as a source of organic matter for heterotrophs. This supports the organic-carbon part of the claim in an autotrophic nitrifying biofilm. The paper does not establish that decay makes nutrient-available nitrogen, sulfur, or selenium available, or demonstrate the claim across inorganic-electron-donor communities generally. (LLM, conf=0.88)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
No references could be resolved yet.
Research works printed but not linked: 5
- [2]2. Amann, R. I. 1995. In situ identification of micro-organisms by whole cell hybridization with rRNA-targeted nucleic acid probes, p. 1-15. In A. D. L. Akkerman, J. D. van Elsas, and F. J. de Bruijn (ed.), Molecular microbial ecology manual. Kluwer Academic Publishers, Dordrecht, The Netherlands.
- [3]3. Barker, D. J., and D. C. Stuckey. 1999. A review of soluble microbial products (SMP) in wastewater treatment systems. Water Res. 33: 3063-3082.
- [22]22. Neef, A. 1997. Anwendung der in situ Einzelzell-Identifizierung von Bakterien zur Populationsanalyse in komplexen mikrobiellen Biozo ¨nosen. Doctoral thesis. Technische Universita ¨t Mu ¨nchen, Munich, Germany.
- [35]35. Rittmann, B. E., and P. L. McCarty. 2001. Microbial kinetics: soluble microbial products, p. 176-178. In B. E. Rittmann and P. L. McCarty (ed.), Environmental biotechnology: principles and applications. McGraw Hill, New York, N.Y.
- [42]42. Wagner, M., R. I. Amann, P. Kampfer, B. Assmus, A. Hartmann, P. Hutzler, N. Springer, and K.-H. Schleifer. 1994. Identification and in situ detection of gram-negative filamentous bacteria in activated sludge. Syst. Appl. Microbiol. 17: 405-417.
Cited by
Sorted by citations
Boltz, J. P., & Rittmann, B. E. (2024) · FEMS Microbiology Ecology · doi:10.1093/femsec/fiae125 · Cited by 10 · see citing works
- Authors
- Okabe, S., Kindaichi, T., & Ito, T.
- Affiliations
- Department of Urban and Environmental Engineering, Graduate School of Engineering, Hokkaido University, Sapporo 060-8628, Japan
- Department of Social and Environmental Engineering, Graduate School of Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashihiroshima 739-8527, Japan
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2005-07
- Volume
- 71
- Issue
- 7
- Pages
- 3987-3994
- ISSN
- 0099-2240
- 1098-5336
- Cited by
- 151
- References
- 0
- Language
- en
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
