The DOI matches its Crossref record.
Cytochrome P450 Alkane Hydroxylases of the CYP153 Family Are Common in Alkane-Degrading Eubacteria Lacking Integral Membrane Alkane Hydroxylases
Open accessAbstract
Several strains that grow on medium-chain-length alkanes and catalyze interesting hydroxylation and epoxidation reactions do not possess integral membrane nonheme iron alkane hydroxylases. Using PCR, we show that most of these strains possess enzymes related to CYP153A1 and CYP153A6, cytochrome P450 enzymes that were characterized as alkane hydroxylases. A vector for the polycistronic coexpression of individual CYP153 genes with a ferredoxin gene and a ferredoxin reductase gene was constructed. Seven of the 11 CYP153 genes tested allowed Pseudomonas putida GPo12 recombinants to grow well on alkanes, providing evidence that the newly cloned P450s are indeed alkane hydroxylases.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Biodegradation of Polyethylene by a Soil Bacterium and AlkB Cloned Recombinant Cell
Gyung Yoon, M., Jeong Jeon, H., & Nam Kim, M. (2012) · Journal of Bioremediation & Biodegradation · doi:10.4172/2155-6199.1000145 · Cited by 304
Biotechnological synthesis of long‐chain dicarboxylic acids as building blocks for polymers
Huf, S., Krügener, S., Hirth, T., Rupp, S., & Zibek, S. (2011) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.201000112 · Cited by 141
Gregson, B. H., Metodieva, G., Metodiev, M. V., & McKew, B. A. (2019) · Environmental Microbiology · doi:10.1111/1462-2920.14620 · Cited by 46
Metadata
- Authors
- van Beilen, J. B., Funhoff, E. G., van Loon, A., Just, A., Kaysser, L., Bouza, M., Holtackers, R., Röthlisberger, M., Li, Z., & Witholt, B.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2006-01
- Volume
- 72
- Issue
- 1
- Pages
- 59-65
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2042673218
- Cited by
- 333
- References
- 0
- FWCI
- 12.91
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Cytochrome P450 Alkane Hydroxylases of the CYP153 Family Are Common in Alkane-Degrading Eubacteria Lacking Integral Membrane Alkane Hydroxylases. https://app.cochat.ai/papers/10.1128/aem.72.1.59-65.2006
- The paper
- van Beilen, J. B., Funhoff, E. G., van Loon, A., Just, A., Kaysser, L., Bouza, M., Holtackers, R., Röthlisberger, M., Li, Z., & Witholt, B. (2006). Cytochrome P450 Alkane Hydroxylases of the CYP153 Family Are Common in Alkane-Degrading Eubacteria Lacking Integral Membrane Alkane Hydroxylases. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.72.1.59-65.2006
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
