The DOI matches its Crossref record.
Rapid Production of Gene Replacement Constructs and Generation of a Green Fluorescent Protein-Tagged Centromeric Marker in Aspergillus nidulans
Open accessAbstract
A method to rapidly generate gene replacement constructs by fusion PCR is described for Aspergillus nidulans . The utility of the approach is demonstrated by green fluorescent protein (GFP) tagging of A. nidulans ndc80 to visualize centromeres through the cell cycle. The methodology makes possible large-scale GFP tagging, promoter swapping, and deletion analysis of A. nidulans .
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Cited by
da Silva Ferreira, M. E., Kress, M. R. V. Z., Savoldi, M., Goldman, M. H. S., Härtl, A., Heinekamp, T., Brakhage, A. A., & Goldman, G. H. (2006) · Eukaryotic Cell · doi:10.1128/ec.5.1.207-211.2006 · Cited by 403
Chou, H., Glory, A., & Bachewich, C. (2011) · Eukaryotic Cell · doi:10.1128/ec.00263-10 · Cited by 20
Metadata
- Authors
- Yang, L., Ukil, L., Osmani, A., Nahm, F., Davies, J., De Souza, C. P. C., Dou, X., Perez-Balaguer, A., & Osmani, S. A.
- Venue
- Eukaryotic Cell
- Publisher
- American Society for Microbiology
- Published
- 2004-10
- Volume
- 3
- Issue
- 5
- Pages
- 1359-1362
- ISSN
- 1535-9778 (ISSN-L)
- 1535-9786
- OpenAlex
- W2155750049
- Cited by
- 239
- References
- 0
- FWCI
- 4.37
- Type
- journal article
- Language
- en
- Open access
- diamond
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- CoChat. (2026). Citation check: Rapid Production of Gene Replacement Constructs and Generation of a Green Fluorescent Protein-Tagged Centromeric Marker in Aspergillus nidulans. https://app.cochat.ai/papers/10.1128/ec.3.5.1359-1362.2004
- The paper
- Yang, L., Ukil, L., Osmani, A., Nahm, F., Davies, J., De Souza, C. P. C., Dou, X., Perez-Balaguer, A., & Osmani, S. A. (2004). Rapid Production of Gene Replacement Constructs and Generation of a Green Fluorescent Protein-Tagged Centromeric Marker in Aspergillus nidulans. Eukaryotic Cell. https://doi.org/10.1128/ec.3.5.1359-1362.2004
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
