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Construction of a urease-negative mutant of Proteus mirabilis: analysis of virulence in a mouse model of ascending urinary tract infection
Open accessAbstract
Proteus mirabilis, a urease-producing uropathogen, causes serious urinary tract infections in humans. To specifically evaluate the contribution of urease to virulence, a mutation was introduced into P. mirabilis HI4320 by homologous recombination. Virulence was assessed in the CBA mouse model of ascending urinary tract infection. Twenty mice each were challenged transurethrally with P. mirabilis HI4320 and its urease-negative derivative (1 x 10(9) to 2 x 10(9) CFU). At 48 h animals were sacrificed and the mean log10 CFU per milliliter of urine (parent, 6.23; mutant, 4.19; P = 0.0014) or per gram of bladder (parent, 6.29; mutant, 4.28; P = 0.0002), left kidney (parent, 4.11; mutant, 1.02; P = 0.00009), and right kidney (parent, 4.11; mutant, 2.43; P = 0.036) were all shown to be significantly different. These data demonstrate a role for urease as a critical virulence determinant for uropathogenic P. mirabilis.
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Metadata
- Authors
- Jones, B. D., Lockatell, C. V., Johnson, D. E., Warren, J. W., & Mobley, H. L.
- Affiliations
- University of Maryland, Baltimore (US)
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 1990-04
- Volume
- 58
- Issue
- 4
- Pages
- 1120-1123
- ISSN
- 0019-9567 (ISSN-L)
- 1098-5522
- PMC
- PMC258592
- OpenAlex
- W1928804885
- Cited by
- 178
- References
- 28
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 6 · 2023: 3 · 2022: 3 · 2021: 10 · 2020: 4 · 2019: 7 · 2018: 5 · 2017: 3 · 2016: 12 · 2015: 6 · 2014: 11 · 2013: 7 · 2012: 4
- FWCI
- 6.71
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Infection and Immunity (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5p01ag004393-09)
- National Institute on Aging (AG-04393)
- National Institute of Allergy and Infectious Diseases (AI23328)
- Topics
- Health Sciences › Medicine › Urology › Urinary Tract Infections Management
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Keywords
- Proteus mirabilis, urease, virulence factors, ascending urinary tract infection, uropathogenic bacteria, mouse model, bladder colonization, kidney colonization
- MeSH terms
- Animals, Female, Mice, Inbred CBA, Mutation, Proteus mirabilis, Urease, Urinary Tract Infections, Virulence, Mice
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