Polymerase chain reaction and synthetic DNA probes: a means of distinguishing the causative agents of syphilis and yaws?
Open accessAbstract
Synthetic DNA probes specific for either the tpf-1 gene of Treponema pallidum subsp. pallidum Nichols or the tyf-1 gene of Treponema pallidum subsp. pertenue CDC 2575 were used for hybridization with in vitro-amplified chromosomal DNAs of 10 different Treponema isolates. tpf-1 and tyf-1 differ only in one nucleotide at residue 123, and three of four syphilis strains were of the Nichols type, whereas five of six yaws strains were of the CDC 2575 type, indicating that the nucleotide at position 123 of the tpf-1 or tyf-1 gene is not a definitive trait for either T. pallidum subspecies.
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Older age as a risk factor for syphilis is suggestive of another confounder to the analysis, namely that existing serologic tests cannot differentiate between syphilis and yaws or other non-venereal treponematoses.
“This is also suggestive of another confounder to our analysis, the fact that existing serologic tests cannot differentiate between syphilis and yaws or other non-venereal treponematoses 28, 29.”
From Audit of antenatal screening for syphilis and HIV in migrant and refugee women on the Thai-Myanmar border: a descriptive study (2015) · cites this paper as [29]
Existing serologic tests cannot differentiate between syphilis and yaws or other non-venereal treponematoses.
“the fact that existing serologic tests cannot differentiate between syphilis and yaws or other non-venereal treponematoses 28, 29.”
From Audit of antenatal screening for syphilis and HIV in migrant and refugee women on the Thai-Myanmar border: a descriptive study (2015) · cites this paper as [29]
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References 19 · Cited by 60
- Authors
- Noordhoek, G. T., Wieles, B., van der Sluis, J. J., & van Embden, J. D.
- Affiliations
- National Institute for Public Health and the Environment (NL)
- Laboratory of Bacteriology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
- ORCID
- Wieles, B
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 1990-06
- Volume
- 58
- Issue
- 6
- Pages
- 2011-2013
- ISSN
- 0019-9567 (ISSN-L)
- 1098-5522
- PMC
- PMC258761
- OpenAlex
- W1891505237
- Cited by
- 60
- References
- 19
- Citations by year
- 2023: 1 · 2021: 1 · 2020: 1 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 2 · 2013: 5 · 2012: 3
- FWCI
- 4.84
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Infection and Immunity (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Rivm Repository (Netherlands National Institute for Public Health and the Environment) (submittedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Syphilis Diagnosis and Treatment
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Life Sciences › Immunology and Microbiology › Parasitology › Vector-borne infectious diseases
- Keywords
- Treponema pallidum subsp. pallidum, Treponema pallidum subsp. pertenue, syphilis, yaws, subspecies differentiation, DNA hybridization, Nichols strain
- MeSH terms
- Base Sequence, DNA, Bacterial, Genes, Bacterial, Molecular Sequence Data, Species Specificity, Syphilis, Treponema pallidum, Yaws, DNA Probes, Polymerase Chain Reaction
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