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Rat monoclonal antibodies against Aspergillus galactomannan
Open accessAbstract
Monoclonal antibodies (MAbs) against Aspergillus fumigatus galactomannan were produced in rats. Seven of them, EB-A1 through EB-A7, were characterized in more detail. They were all immunoglobulin M antibodies, reacting in an indirect enzyme-linked immunosorbent assay with purified A. fumigatus galactomannan, with avidity constants of between 2 x 10(9) and 5 x 10(9)/M. Enzyme-linked immunosorbent assay inhibition experiments with modified galactomannan and synthetic oligomers of beta (1----5)galactofuranose demonstrated that the MAbs bound to an epitope located on the beta(1----5)galactofuranose-containing side chains of the galactomannan molecule. An identical or similar epitope also seemed to be present in other fungi. Immunofluorescence and immunoelectron microscopy experiments with EB-A2 revealed the presence of the antigen in the fungal wall and inside the cell. Immunoblotting experiments demonstrated that the epitope recognized by the MAbs was a common oligosaccharide moiety of a wide range of intracellular and extracellular glycoproteins in A. fumigatus. The characteristics of the MAbs justify their use in the diagnosis of invasive aspergillosis by antigen detection.
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“The method currently used is a sandwich ELISA based on a very efficient EB-A2 rat monoclonal antibody able to detect 0.5 to 1 ng of the galactofuran epitope (107, 108).”
From Aspergillus fumigatus and Aspergillosis in 2019 (2019) · cites this paper as [107]
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Metadata
- Authors
- Stynen, D., Sarfati, J., Goris, A., Prévost, M. C., Lesourd, M., Kamphuis, H., Darras, V, & Latgé, J. P.
- Affiliations
- Sanofi (Belgium) (BE)
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 1992-06
- Volume
- 60
- Issue
- 6
- Pages
- 2237-2245
- ISSN
- 0019-9567 (ISSN-L)
- 1098-5522
- PMC
- PMC257149
- OpenAlex
- W1915036617
- Cited by
- 267
- References
- 47
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 3 · 2023: 4 · 2022: 7 · 2021: 7 · 2020: 5 · 2019: 19 · 2018: 8 · 2017: 8 · 2016: 8 · 2015: 5 · 2014: 7 · 2013: 9 · 2012: 11
- FWCI
- 10.53
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Infection and Immunity (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Socio-Environmental Systems Modeling (acceptedVersion)
- Lirias (submittedVersion)
- Rivm Repository (Netherlands National Institute for Public Health and the Environment) (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Infectious Diseases › Fungal Infections and Studies
- Keywords
- Aspergillus fumigatus, galactomannan, monoclonal antibodies, fungal cell wall, antigen detection, fungal glycoproteins, epitope mapping, IgM
- MeSH terms
- Animals, Antibodies, Monoclonal, Epitopes, Antigens, Fungal, Aspergillus, Cross Reactions, Fluorescent Antibody Technique, Galactose, Mannans, Immunoblotting, Rats
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