Vibrio cholerae iron transport systems: roles of heme and siderophore iron transport in virulence and identification of a gene associated with multiple iron transport systems
Open accessAbstract
Vibrio cholerae iron transport mutants were tested for their ability to cause disease in an infant mouse model. The mice were challenged with either the wild-type strain, a vibriobactin synthesis mutant, a heme utilization mutant, or double mutants containing both the vibriobactin synthesis defect and the heme utilization defect. When mice were challenged with 10(7) bacteria, the ability of the double mutant to survive in the intestines was greatly reduced and that of the heme utilization mutant was slightly reduced compared with that of the wild type or the vibriobactin synthesis mutant. When the inoculum size was reduced 10-fold, all of the iron transport mutants failed to colonize the intestines and failed to cause diarrhea in the mice, whereas the wild-type strain was not cleared and elicited a diarrheal response. These data indicate that disruption of either the heme utilization or the vibriobactin uptake system reduces the ability of V. cholerae to cause disease. One of the heme utilization mutants, DHH1, was found to be defective also in utilization of vibriobactin and ferrichrome, mimicking the Escherichia coli TonB- phenotype. This mutant was the least virulent of the iron transport mutants tested. Transformation of DHH1 with the recombinant plasmid pHUT4 restored the abilities to use hemin, vibriobactin, and ferrichrome as iron sources, suggesting that pHUT4 encodes a gene(s) involved globally in the iron transport systems. Hybridization of Vibrio DNA with the V. ch…
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“The colour change from blue to yellow was observed by measuring the absorbance at 600 nm, using uninoculated broth without reagent as the blank and siderophore desferrioxamine (up to 50 µM) as the standard (Henderson and Payne, 1994).”
- Authors
- Henderson, D. P., & Payne, S. M.
- Affiliations
- The University of Texas at Austin (US)
- ORCID
- Payne, S M
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 1994-11
- Volume
- 62
- Issue
- 11
- Pages
- 5120-5125
- ISSN
- 0019-9567 (ISSN-L)
- 1098-5522
- PMC
- PMC303233
- OpenAlex
- W1943555456
- Cited by
- 162
- References
- 0
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 3 · 2023: 4 · 2022: 5 · 2021: 2 · 2019: 5 · 2018: 1 · 2017: 7 · 2016: 6 · 2015: 11 · 2014: 3 · 2013: 7 · 2012: 10
- FWCI
- 2.99
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Infection and Immunity (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Vibrio bacteria research studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Keywords
- Vibrio cholerae, iron transport, heme utilization, siderophore uptake, ferrichrome, virulence, intestinal colonization, diarrhea, TonB-dependent receptors, Vibrio parahaemolyticus
- MeSH terms
- Animals, Catechols, DNA, Bacterial, Genes, Bacterial, Heme, Intestines, Iron, Mice, Inbred BALB C, Oxazoles, Sequence Homology, Nucleic Acid, Vibrio cholerae, Restriction Mapping, Siderophores, Mice
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