RNA Encoding the MPT83 Antigen Induces Protective Immune Responses against Mycobacterium tuberculosis Infection
Open accessAbstract
We have previously demonstrated that vaccination of mice with plasmid DNA vectors expressing immunodominant mycobacterial genes induced cellular immune responses and significant protection against challenge with Mycobacterium tuberculosis. We demonstrate here, using in vitro-synthesized RNA, that vaccination with DNA or RNA constructs expressing the M. tuberculosis MPT83 antigen are capable of inducing specific humoral and T-cell immune responses and confer modest but significant protection against M. tuberculosis challenge in mice. This is the first report of protective immunity conferred against intracellular bacteria by an RNA vaccine. This novel approach avoids some of the drawbacks of DNA vaccines and illustrates the potential for developing new antimycobacterial immunization strategies.
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Immunization with an mRNA vaccine based on the full-length MPT83 antigen triggered production of specific antituberculosis antibodies.
“Immunization with an mRNA vaccine based on the full-length MPT83 antigen triggered production of specific antituberculosis antibodies [35].”
From Untranslated Region Sequences and the Efficacy of mRNA Vaccines against Tuberculosis (2024) · cites this paper as [35]
There are two experimental studies that demonstrated efficacy of mRNA vaccines against tuberculosis.
“There are two experimental studies that demonstrated efficacy of the mRNA vaccines against tuberculosis [34,35].”
From Untranslated Region Sequences and the Efficacy of mRNA Vaccines against Tuberculosis (2024) · cites this paper as [35]
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Induction of virus‐specific cytotoxic T lymphocytes <i>in vivo</i> by liposome‐entrapped mRNA
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Research works printed but not linked: 5
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Untranslated Region Sequences and the Efficacy of mRNA Vaccines against Tuberculosis
Reshetnikov, V., Terenin, I., Shepelkova, G., Yeremeev, V., Kolmykov, S., Nagornykh, M., Kolosova, E., Sokolova, T., Zaborova, O., Kukushkin, I., et al. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25020888 · Cited by 33 · see citing works
Advancing mRNA technologies for therapies and vaccines: An African context
Kairuz, D., Samudh, N., Ely, A., Arbuthnot, P., & Bloom, K. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2022.1018961 · Cited by 25 · see citing works
- Authors
- Xue, T., Stavropoulos, E., Yang, M., Ragno, S., Vordermeier, M., Chambers, M., Hewinson, G., Lowrie, D. B., Colston, M. J., & Tascon, R. E.
- Affiliations
- National Institute for Medical Research (GB)
- Central Veterinary Laboratory, Addlestone, Surrey, United Kingdom
- Corresponding author
- Tascon, Ricardo E.
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 2004-11
- Volume
- 72
- Issue
- 11
- Pages
- 6324-6329
- ISSN
- 0019-9567 (ISSN-L)
- 1098-5522
- PMC
- PMC523008
- OpenAlex
- W2102919932
- Cited by
- 73
- References
- 1
- Citations by year
- 2026: 2 · 2025: 11 · 2024: 8 · 2023: 12 · 2022: 12 · 2021: 3 · 2020: 1 · 2018: 2 · 2015: 1 · 2014: 2 · 2013: 1 · 2012: 3
- FWCI
- 1.58
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Infection and Immunity (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Open Research (University of Surrey) (submittedVersion)
- Surrey Research Insight Open Access (The University of Surrey) (submittedVersion)
- ORCA Online Research @Cardiff (Cardiff University) (submittedVersion)
- Funders
- World Health Organization
- Topics
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Life Sciences › Immunology and Microbiology › Immunology › Immune responses and vaccinations
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Keywords
- Mycobacterium tuberculosis, RNA vaccines, DNA vaccine, protective immunity, humoral immune response, T cell response, intracellular bacterial infection, tuberculosis vaccination, plasmid vectors
- MeSH terms
- Amino Acid Sequence, Animals, Antibodies, Bacterial, Antigens, Bacterial, Bacterial Proteins, Cell Line, Female, Cricetinae, Membrane Proteins, Mice, Inbred BALB C, Molecular Sequence Data, Mycobacterium tuberculosis, RNA-Dependent RNA Polymerase, RNA, Bacterial, RNA, Messenger, Sindbis Virus, T-Lymphocytes, Transfection, Tuberculosis, Pulmonary, Vaccination, Vaccines, DNA, Tuberculosis Vaccines, Mice
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