The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
The Azospirillum brasilense Che1 Chemotaxis Pathway Controls Swimming Velocity, Which Affects Transient Cell-to-Cell Clumping
Open accessAbstract
The Che1 chemotaxis-like pathway of Azospirillum brasilense contributes to chemotaxis and aerotaxis, and it has also been found to contribute to regulating changes in cell surface adhesive properties that affect the propensity of cells to clump and to flocculate. The exact contribution of Che1 to the control of chemotaxis and flocculation in A. brasilense remains poorly understood. Here, we show that Che1 affects reversible cell-to-cell clumping, a cellular behavior in which motile cells transiently interact by adhering to one another at their nonflagellated poles before swimming apart. Clumping precedes and is required for flocculation, and both processes appear to be independently regulated. The phenotypes of a ΔaerC receptor mutant and of mutant strains lacking cheA1, cheY1, cheB1, or cheR1 (alone or in combination) or with che1 deleted show that Che1 directly mediates changes in the flagellar swimming velocity and that this behavior directly modulates the transient nature of clumping. Our results also suggest that an additional receptor(s) and signaling pathway(s) are implicated in mediating other Che1-independent changes in clumping identified in the present study. Transient clumping precedes the transition to stable clump formation, which involves the production of specific extracellular polysaccharides (EPS); however, production of these clumping-specific EPS is not directly controlled by Che1 activity. Che1-dependent clumping may antagonize motility and prevent chemotaxis, thereby maintaining cells in a metabolically favorable niche.
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“Chemotaxis also plays a role in the autoaggregation of Azospirillum brasiliense, a plant-associated soil bacterium [99]. Here, the chemotactic signal transduction pathway Che1 increases swimming velocity with changes in aeration conditions; mutants defective in Che1 do not alter their swimming speed and do not detach from early, reversibly formed clumps. This in turn leads to formation of larger fl”
From Bacterial autoaggregation (2018) · cites this paper as [100]
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Trunk, T., Bacterial Cell Surface Group, S. F. G. A. E. B., S. Khalil, H., & C. Leo, J. (2018) · AIMS Microbiology · doi:10.3934/microbiol.2018.1.140 · Cited by 444
“This in turn leads to formation of larger flocs stabilised by exopolysaccharides [100] These examples demonstrate that autoaggregation is not always simply a passive phenomenon but can be an active process, where cells expend energy to move along chemoattractant gradients to join forming aggregates.”
BackgroundChemotaxis towards autoinducer 2 mediates autoaggregation in Escherichia coli
Laganenka, L., Colin, R., & Sourjik, V. (2016) · Nature Communications · doi:10.1038/ncomms12984 · Cited by 218
Metadata
- Authors
- Bible, A., Russell, M. H., & Alexandre, G.
- Affiliations
- University of Tennessee at Knoxville (US)
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2012-07
- Volume
- 194
- Issue
- 13
- Pages
- 3343-3355
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC3434747
- OpenAlex
- W2126751599
- Cited by
- 50
- References
- 41
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 5 · 2020: 3 · 2019: 6 · 2018: 4 · 2017: 5 · 2016: 4 · 2015: 6 · 2014: 2 · 2013: 6 · 2012: 1
- FWCI
- 2.89
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Bacteriology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Keywords
- Azospirillum brasilense, aerotaxis, flocculation, cell adhesion, exopolysaccharides, chemotaxis receptor, bacterial motility
- MeSH terms
- Bacterial Adhesion, Bacterial Proteins, Cell Membrane, Chemotaxis, Mutation, Surface Properties, Signal Transduction, Azospirillum brasilense, Gene Expression Regulation, Bacterial
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