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Dissection and Reconstitution Provide Insights into Electron Transport in the Membrane-Bound Aldehyde Dehydrogenase Complex of Gluconacetobacter diazotrophicus
Open accessAbstract
Two membrane-bound enzymes of acetic acid bacteria, pyrroloquinoline quinone-dependent alcohol dehydrogenase and molybdopterin-dependent aldehyde dehydrogenase (ALDH), are responsible for vinegar production. Upon the oxidation of acetaldehyde, ALDH reduces ubiquinone in the cytoplasmic membrane.
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“The first step in the ethanol oxidation process involves the conversion of ethanol to acetaldehyde. This reaction is catalyzed by pyrroloquinoline quinone-dependent alcohol dehydrogenase (PQQ-ADH), which oxidizes ethanol to acetaldehyde, resulting in the reduction of PQQ to PQQH2. The second step entails the further oxidation of acetaldehyde to acetic acid (CH3COOH). This reaction is mediated by”
From The Metabolic Pathways of Yeast and Acetic Acid Bacteria During Fruit Vinegar Fermentation and Their Influence on Flavor Development (2025) · cites this paper as [63]
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Metadata
- Authors
- Miah, R., Nina, S., Murate, T., Kataoka, N., Matsutani, M., Ano, Y., Matsushita, K., & Yakushi, T.
- Affiliations
- Yamaguchi University (JP)
- Tokyo University of Agriculture (JP)
- Ehime University (JP)
- Corresponding author
- Yakushi, Toshiharu
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2022-03-15
- Volume
- 204
- Issue
- 3
- Pages
- e0055821-e0055821
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC8923213
- OpenAlex
- W4207019399
- Cited by
- 4
- References
- 40
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 1
- FWCI
- 0.24
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Bacteriology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- MEXT | Japan Society for the Promotion of Science (23580115)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Enzyme Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Metalloenzymes and iron-sulfur proteins
- Keywords
- aldehyde dehydrogenase, Gluconacetobacter diazotrophicus, electron transport, ubiquinone reduction, vinegar fermentation
- MeSH terms
- Acetaldehyde, Alcohol Dehydrogenase, Aldehyde Dehydrogenase, Aldehydes, Cytochromes, Electron Transport, Ubiquinone, Acetic Acid, Gluconacetobacter, PQQ Cofactor
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