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Isolation and Properties of a Fluorescent Compound, Factor 420 , from Methanobacterium Strain M.o.H
Open accessAbstract
A new fluorescent compound, factor 420 (F 420 ), which is involved in the hydrogen metabolism of hydrogen-grown Methanobacterium strain M.o.H. has been isolated and purified. Acid hydrolysis of this compound with 6 m HCl for 24 hr releases a ninhydrin-positive compound (glutamic acid), an acid-stable chromophore, phosphate, and an ether-soluble phenolic component. Factor 420 may be reduced by either sodium dithionite or sodium borohydride at p H 7.3 with concomitant loss of its fluorescence and its major absorption peak at 420 nm. Crude cell-free extracts of strain M.o.H. reduce F 420 only under a hydrogen atmosphere. F 420 is photolabile aerobically in neutral and basic solutions, whereas the acid-stable chromophore is not photolabile under these conditions. An approximate molecular weight of 630 ± 8% for F 420 was determined by Sephadex G-25 chromatography. At the present time, F 420 is proposed as a trivial name for the unknown fluorescent compound because of its strong absorption maximum of 420 nm at p H 7.
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Cited by
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Thauer, R. K., Jungermann, K., & Decker, K. (1977) · Bacteriological Reviews · doi:10.1128/br.41.1.100-180.1977 · Cited by 4,146
Phylogenetic structure of the prokaryotic domain: The primary kingdoms
Woese, C. R., & Fox, G. E. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.11.5088 · Cited by 4,131
Anaerobic biotechnology for industrial wastewater treatment
Speece, R. E. (1983) · Environmental Science & Technology · doi:10.1021/es00115a001 · Cited by 721
Isolation of Extremely Thermophilic Sulfate Reducers: Evidence for a Novel Branch of Archaebacteria
Stetter, K. O., Lauerer, G., Thomm, M., & Neuner, A. (1987) · Science · doi:10.1126/science.236.4803.822 · Cited by 317
Growth and Methanogenesis by Methanosarcina Strain 227 on Acetate and Methanol
Smith, M. R., & Mah, R. A. (1978) · Applied and Environmental Microbiology · doi:10.1128/aem.36.6.870-879.1978 · Cited by 259
Metadata
- Authors
- Cheeseman, P., Toms-Wood, A., & Wolfe, R. S.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1972-10
- Volume
- 112
- Issue
- 1
- Pages
- 527-531
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W1904943041
- Cited by
- 221
- References
- 0
- FWCI
- 2.22
- Type
- journal article
- Language
- en
- Open access
- green
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- Cheeseman, P., Toms-Wood, A., & Wolfe, R. S. (1972). Isolation and Properties of a Fluorescent Compound, Factor 420 , from Methanobacterium Strain M.o.H. Journal of Bacteriology. https://doi.org/10.1128/jb.112.1.527-531.1972
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