The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Rapid procedure for detection and isolation of large and small plasmids
Open accessAbstract
Procedures are described for the detection and isolation of plasmids of various sizes (2.6 to 350 megadaltons) that are harbored in species of Agrobacterium, Rhizobium, Escherichia, Salmonella, Erwinia, Pseudomonas, and Xanthomonas. The method utilized the molecular characteristics of covalently closed circular deoxyribonucleic acid (DNA) that is released from cells under conditions that denature chromosomal DNA by using alkaline sodium dodecyl sulfate (pH 12.6) at elevated temperatures. Proteins and cell debris were removed by extraction with phenol-chloroform. Under these conditions chromosomal DNA concentrations were reduced or eliminated. The clarified extract was used directly for electrophoretic analysis. These procedures also permitted the selective isolation of plasmid DNA that can be used directly in nick translation, restriction endonuclease analysis, transformation, and DNA cloning experiments.
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Citations
Cites
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Cited by
Straley, S. C., & Bowmer, W. S. (1986) · Infection and Immunity · doi:10.1128/iai.51.2.445-454.1986 · Cited by 367
Endophytic Colonization of Rice by a Diazotrophic Strain of Serratia marcescens
Gyaneshwar, P., James, E. K., Mathan, N., Reddy, P. M., Reinhold-Hurek, B., & Ladha, J. K. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.8.2634-2645.2001 · Cited by 357
Eckert, C., Gautier, V., Saladin-Allard, M., Hidri, N., Verdet, C., Ould-Hocine, Z., Barnaud, G., Delisle, F., Rossier, A., Lambert, T., et al. (2004) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.48.4.1249-1255.2004 · Cited by 278
Nassif, X., & Sansonetti, P. J. (1986) · Infection and Immunity · doi:10.1128/iai.54.3.603-608.1986 · Cited by 277
Feasey, N. A., Hadfield, J., Keddy, K. H., Dallman, T. J., Jacobs, J., Deng, X., Wigley, P., Barquist, L., Langridge, G. C., Feltwell, T., et al. (2016) · Nature Genetics · doi:10.1038/ng.3644 · Cited by 217
Metadata
- Authors
- Kado, C. I., & Liu, S. T.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1981-03
- Volume
- 145
- Issue
- 3
- Pages
- 1365-1373
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W1690413094
- Cited by
- 2,809
- References
- 1
- FWCI
- 46.87
- Type
- journal article
- Language
- en
- Open access
- green
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- Kado, C. I., & Liu, S. T. (1981). Rapid procedure for detection and isolation of large and small plasmids. Journal of Bacteriology. https://doi.org/10.1128/jb.145.3.1365-1373.1981
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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